Molecular and morphological study of SDF-1 in tumor proliferation of pituitary adenoma

SDF-1在垂体腺瘤增殖中的分子及形态学研究

• 批准号: 20591726
• 负责人: YOSHIDA Daizo
• 金额: $ 2.91万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591726/
• 关键词: 低酸素; SDF-1; CXCR4; 下垂体腺腫; インターフェロン誘導性膜貫通蛋白質; 細胞内シグナル; cDNA microarray; 細胞浸潤; 血管新生; Gremlin; tissue microarray; siRNA; CXCR7

In the present study, we aimed to develop a novel transfection method for short interference RNA (siRNA) to employ nanotube by the surfactant activity.A6K consisting of six alanine residues and hydrophilic head, lysine, was compared with conventional cationic transfectant reagent, siFECTOR and Lipofectamine 2000. Cyto-toxity for human rat pituitary adenoma cell line, AtT-20 or GH3 was examined with MTS assay. Transfection efficiency was analyzed with FITC-labelled siRNA targeting SDF-1 receptor mRNA with fluorescent activity and microscopy. Ultra-structure of A6K was observed with electron microscopy.Non-cytotoxic level of A6K (IC50, 320.8 ・g/ml) was significantly higher than that of siFECTOR ((IC50, 35.6 ・g/ml) and Lipofectamine 2000 (IC50, 22.4 ・g/ml). Transfection efficiency for the siRNA was elevated in a dose- and time- dependent fashion. Relative expression of SDF-1 and its receptor, CXCR4 and CXCR7 mRNA to ・-actin was reduced in a dose-dependent manner and hypoxia in a real-time RT-PCR study. Ultra-structure of A6K was transformed to micelle formation when siRNA was mixed.Lipid-like self assembling peptide, A6K includes genes in the micelle due to hydrophilic tail. This transfection is a novel and stable method with lower cytotoxity. In conclusion, SDF-1 and its receptor-mediated cell signal pathway is enhanced in hypoxic condition.

在介绍研究中，我们旨在开发一种新型的翻译方法，用于通过基础活动使用纳米管，以纳米管的基础活性。由六个丙氨酸残留物和赖氨酸组成的A6K与常规阳离子阳离子的转化试剂，Sifater和Lipofectamine 2000。 MTS分析。用FITC标记的siRNA靶向具有荧光活性和显微镜的FITC标记的siRNA分析转染效率。用电子显微镜观察到A6K的超结构。非毒性水平的A6K（IC50，320.8 g/ml）显着高于Sifatecter（（（IC50，35.6 ・ g/ml）和脂肪触及胺2000（IC50，IC50，IC50，22.4），g/ml-g/ml-g/ml-rippitients。时尚。在实时的RT-PCR研究中，以剂量依赖的方式降低了SDF-1及其受体的表达。稳定的细胞毒性稳定方法，总结为SDF-1及其受体介导的细胞信号途径在低氧条件下增强了。

项目成果

Clinical clerkship course for medical students on lumbar puncture usingsimulators.

使用模拟器为医学生提供腰椎穿刺临床见习课程。

• 发表时间: 2012
• 期刊: J Nippon Med Sch.
• 作者: Adachi K;Yoshimura A;Aso R;Miyashita T;Yoshida D;Teramoto A;Shimura T.
• 通讯作者: Shimura T.

In celebration of NMC's 50th anniversary.

庆祝 NMC 成立 50 周年。

• 发表时间: 2010
• 期刊: Neurol Med Chir(Tokyo)
• 作者: Ota T;Kawai K;Kamada K;Kin T;Saito N;川原信隆;高野晋吾;A Teramoto
• 通讯作者: A Teramoto

Intracerebral hemorrhage caused by a neoplastic aneurysm from pleomorphic lung carcinoma.

多形性肺癌肿瘤性动脉瘤引起的脑出血。

• 发表时间: 2009
• 期刊: Neurol Med Chir (Tokyo).
• 作者: Nomura R;Yoshida D;Kim K;Kobayashi S;Teramoto A.
• 通讯作者: Teramoto A.

人工ペプタイドを用いた新しい遺伝子導入法

使用人工肽的新基因转移方法

• 发表时间: 2009
• 作者: 吉田大蔵;纐纈健太;寺本明
• 通讯作者: 寺本明

A novel transfection method for short interference RNA with lipid-like self-assembling nanotube, A6K

类脂质自组装纳米管A6K转染短干扰RNA的新方法

• 发表时间: 2010
• 作者: D Yoshida;他
• 通讯作者: 他