Molecular and morphological study of SDF-1 in tumor proliferation of pituitary adenoma

SDF-1在垂体腺瘤增殖中的分子及形态学研究

• 批准号: 20591726
• 负责人: YOSHIDA Daizo
• 金额: $ 2.91万
• 依托单位: Nippon Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591726/
• 关键词: 低酸素; SDF-1; CXCR4; 下垂体腺腫; インターフェロン誘導性膜貫通蛋白質; 細胞内シグナル; cDNA microarray; 細胞浸潤; 血管新生; Gremlin; tissue microarray; siRNA; CXCR7

In the present study, we aimed to develop a novel transfection method for short interference RNA (siRNA) to employ nanotube by the surfactant activity.A6K consisting of six alanine residues and hydrophilic head, lysine, was compared with conventional cationic transfectant reagent, siFECTOR and Lipofectamine 2000. Cyto-toxity for human rat pituitary adenoma cell line, AtT-20 or GH3 was examined with MTS assay. Transfection efficiency was analyzed with FITC-labelled siRNA targeting SDF-1 receptor mRNA with fluorescent activity and microscopy. Ultra-structure of A6K was observed with electron microscopy.Non-cytotoxic level of A6K (IC50, 320.8 ・g/ml) was significantly higher than that of siFECTOR ((IC50, 35.6 ・g/ml) and Lipofectamine 2000 (IC50, 22.4 ・g/ml). Transfection efficiency for the siRNA was elevated in a dose- and time- dependent fashion. Relative expression of SDF-1 and its receptor, CXCR4 and CXCR7 mRNA to ・-actin was reduced in a dose-dependent manner and hypoxia in a real-time RT-PCR study. Ultra-structure of A6K was transformed to micelle formation when siRNA was mixed.Lipid-like self assembling peptide, A6K includes genes in the micelle due to hydrophilic tail. This transfection is a novel and stable method with lower cytotoxity. In conclusion, SDF-1 and its receptor-mediated cell signal pathway is enhanced in hypoxic condition.

在本研究中，我们旨在利用表面活性剂的活性，开发一种新的转染短干扰RNA （siRNA）的方法。A6K由6个丙氨酸残基和亲水头赖氨酸组成，与常规阳离子转染试剂siFECTOR和Lipofectamine 2000进行了比较。用MTS法检测人大鼠垂体腺瘤细胞株at -20和GH3的细胞毒性。用fitc标记的siRNA靶向SDF-1受体mRNA，用荧光活性和显微镜分析转染效率。用电镜观察了A6K的超微结构。A6K的非细胞毒水平（IC50, 320.8·g/ml）显著高于siFECTOR （IC50, 35.6·g/ml）和Lipofectamine 2000 （IC50, 22.4·g/ml）。siRNA的转染效率以剂量和时间依赖的方式提高。在实时RT-PCR研究中，SDF-1及其受体CXCR4和CXCR7 mRNA对-actin的相对表达以剂量依赖性和缺氧方式降低。当siRNA混合时，A6K的超结构转化为胶束形成。类脂自组装肽，A6K由于亲水性尾巴在胶束中包含基因。这是一种新颖、稳定、细胞毒性低的转染方法。综上所述，缺氧条件下SDF-1及其受体介导的细胞信号通路增强。

项目成果

Selective Posterior Decompression of the Cervical Spine

颈椎选择性后路减压

• DOI: 10.2176/nmc.51.108
• 发表时间: 2011
• 期刊: Neurologia medico-chirurgica
• 影响因子: 1.9
• 作者: Kim K;Isu T;Sugawara A;Matsumoto R;Isobe M;Morimoto D;Mishina M;Kobayashi S;Yoshida D;Teramoto A
• 通讯作者: Teramoto A

Clinical clerkship course for medical students on lumbar puncture usingsimulators.

使用模拟器为医学生提供腰椎穿刺临床见习课程。

• 发表时间: 2012
• 期刊: J Nippon Med Sch.
• 作者: Adachi K;Yoshimura A;Aso R;Miyashita T;Yoshida D;Teramoto A;Shimura T.
• 通讯作者: Shimura T.

Transcranial Doppler Ultrasonography for Diagnosis of Cerebral Vasospasm After Aneurysmal Subarachnoid Hemorrhage: Mean Blood Flow Velocity Ratio of the Ipsilateral and Contralateral Middle Cerebral Arteries

• DOI: 10.1227/neu.0b013e318222dc4c
• 发表时间: 2011-10-01
• 期刊: NEUROSURGERY
• 影响因子: 4.8
• 作者: Nakae, Ryuta;Yokota, Hiroyuki;Teramoto, Akira
• 通讯作者: Teramoto, Akira

Incidental Detection of Thyroid Nodules at Magnetic Resonance Imaging of the Cervical Spine

• DOI: 10.2176/nmc.53.77
• 发表时间: 2013-02-01
• 期刊: NEUROLOGIA MEDICO-CHIRURGICA
• 影响因子: 1.9
• 作者: Kim, Kyongsong;Emoto, Naoya;Teramoto, Akira
• 通讯作者: Teramoto, Akira

Early computed tomography signs as early predictors of hemorrhagic transformation under heparinization in patients with cardiogenic embolism.

早期计算机断层扫描迹象是心源性栓塞患者肝素化下出血转化的早期预测因子。

• 发表时间: 2012
• 期刊: Geriatr Gerontol Int.
• 作者: Terao T;Mishina M;Takumi I;Komaba Y;Mizunari T;Kobayashi S;Yoshida D;Teramoto A.
• 通讯作者: Teramoto A.