The Development of the regenerated mucosal epithelial transplantation for immunomodulation and ocular surface reconstruction.

我们成功制备了可用于眼表重建的培养角膜上皮片和口腔粘膜上皮片。这些上皮片在基底区表达p75，表明细胞增殖的潜力很大。异位存活的角膜口腔黏膜上皮既不表达PAX6基因，也不表达K1和K10。然而，这些细胞表达K4、K3和K13，但不表达K12。移植后存活的口腔黏膜上皮在角膜上的表型与体内的口腔黏膜上皮相同，提示培养的口腔黏膜上皮不会转分化为角膜上皮。术后早期诱导周围新生血管形成和淋巴生成。体内共聚焦显微镜分析显示组织良好的分层上皮包含适当的细胞密度的基底细胞和扁平的顶端细胞。抗vegf治疗可以抑制眼表血管的进展，并控制存活上皮细胞的表型。

We have successfully generated cultivated corneal epithelial sheet and oral mucosal epithelial sheet which are applicable for ocular surface reconstruction. These epithelial sheets expressed p75 in the basal region suggesting the high potential for cell proliferation. Ectopicallysurvived oral mucosal epithelium on the cornea expressed neither PAX6 gene nor K1 and K10. However,these cells expressed K4, K3 and K13, but not K12. The phenotype of surviving transplanted oral mucosal epithelium on the cornea was the same as in vivo oral mucosal epithelium, suggesting that cultivated oral mucosal epithelium does not transdifferentiate into corneal epithelium. Peripheralneovascularization and lymphanogenesis were induced in early post-operative phase. In vivo confocal microscopic analysis revealed well-organized stratified epithelium containing the proper cell density of basal cells and flattened apical cells. Anti-VEGF treatment could inhibit the progression of vessels and control the phenotype of surviving epithelial cells on the ocular surface.