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The Development of the regenerated mucosal epithelial transplantation for immunomodulation and ocular surface reconstruction.

用于免疫调节和眼表重建的再生粘膜上皮移植的发展。

基本信息

批准号：
20592054
负责人：
INATOMI Tsutomu
金额：
$ 3万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2010
项目状态：
已结题

项目摘要

We have successfully generated cultivated corneal epithelial sheet and oral mucosal epithelial sheet which are applicable for ocular surface reconstruction. These epithelial sheets expressed p75 in the basal region suggesting the high potential for cell proliferation. Ectopicallysurvived oral mucosal epithelium on the cornea expressed neither PAX6 gene nor K1 and K10. However,these cells expressed K4, K3 and K13, but not K12. The phenotype of surviving transplanted oral mucosal epithelium on the cornea was the same as in vivo oral mucosal epithelium, suggesting that cultivated oral mucosal epithelium does not transdifferentiate into corneal epithelium. Peripheralneovascularization and lymphanogenesis were induced in early post-operative phase. In vivo confocal microscopic analysis revealed well-organized stratified epithelium containing the proper cell density of basal cells and flattened apical cells. Anti-VEGF treatment could inhibit the progression of vessels and control the phenotype of surviving epithelial cells on the ocular surface.

我们已成功制备出适用于眼表重建的培养角膜上皮片和口腔粘膜上皮片。这些上皮片在基底区域表达 p75，表明细胞增殖的潜力很高。角膜上异位存活的口腔粘膜上皮既不表达PAX6基因，也不表达K1和K10。然而，这些细胞表达K4、K3和K13，但不表达K12。角膜上存活的移植口腔粘膜上皮的表型与体内口腔粘膜上皮相同，表明培养的口腔粘膜上皮不会转分化为角膜上皮。术后早期诱导外周新生血管和淋巴生成。体内共聚焦显微镜分析显示组织良好的复层上皮含有适当的细胞密度的基底细胞和扁平的顶端细胞。抗 VEGF 治疗可以抑制血管的进展并控制眼表面存活上皮细胞的表型。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Genomic aberrations and cellular heterogeneity in SV40-immortalized human corneal epithelial cells.

DOI：
10.1167/iovs.08-2239
发表时间：
2009-02
期刊：
Investigative ophthalmology & visual science
影响因子：
4.4
作者：
K. Yamasaki;S. Kawasaki;R. Young;H. Fukuoka;H. Tanioka;Mina Nakatsukasa;A. Quantock;S. Kinoshita-
通讯作者：
K. Yamasaki;S. Kawasaki;R. Young;H. Fukuoka;H. Tanioka;Mina Nakatsukasa;A. Quantock;S. Kinoshita-

Surgery of the conjunctiva.

结膜手术。