The Development of the regenerated mucosal epithelial transplantation for immunomodulation and ocular surface reconstruction.

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We have successfully generated cultivated corneal epithelial sheet and oral mucosal epithelial sheet which are applicable for ocular surface reconstruction. These epithelial sheets expressed p75 in the basal region suggesting the high potential for cell proliferation. Ectopicallysurvived oral mucosal epithelium on the cornea expressed neither PAX6 gene nor K1 and K10. However,these cells expressed K4, K3 and K13, but not K12. The phenotype of surviving transplanted oral mucosal epithelium on the cornea was the same as in vivo oral mucosal epithelium, suggesting that cultivated oral mucosal epithelium does not transdifferentiate into corneal epithelium. Peripheralneovascularization and lymphanogenesis were induced in early post-operative phase. In vivo confocal microscopic analysis revealed well-organized stratified epithelium containing the proper cell density of basal cells and flattened apical cells. Anti-VEGF treatment could inhibit the progression of vessels and control the phenotype of surviving epithelial cells on the ocular surface.