Visual rehabilitation and biological analysis of cultivated oral mucosal epithelium and reconstructed ocular surface

培养的口腔粘膜上皮和重建眼表的视觉康复和生物学分析

• 批准号: 23592614
• 负责人: INATOMI Tsutomu
• 金额: $ 3.33万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592614/
• 关键词: 再生医療; 角膜上皮; 口腔粘膜上皮; 眼表面再建; 細胞分化; 血管新生; 新生血管; 新生血管因子

Ocular surface reconstruction using tissue-engineered epithelial transplantation is a new therapeutic modality to overcome severe ocular surface disorders. Oral mucosal epithelium has a high proliferative capacity and similarity with ocular surface suggesting the possible use of alternative epithelium. In this study we have demonstrated that cultivated oral mucosal epithelial transplantation improved the clinical outcome. Biological characters and gene expression patterns of ocular surface epithelium and oral mucosal epithelium were analyzed. Cultivation of oral mucosal epithelium induced the different gene expression profiles, however, the phenotype of surviving transplanted oral mucosal epithelium was the same as in vivo oral mucosal epithelium suggesting that cultivated oral mucosal epithelium does not transdifferentiate into corneal epithelium. These basic findings were expected to contribute for further understanding for ocular surface mechanisms and developing a new therapy.

利用组织工程上皮移植进行眼表重建是治疗严重眼表疾病的一种新方法。口腔粘膜上皮具有高增殖能力，与眼表相似，提示可能使用替代上皮。在这项研究中，我们已经证明，培养的口腔粘膜上皮移植改善了临床结果。分析眼表上皮和口腔粘膜上皮的生物学特性和基因表达谱。培养的口腔粘膜上皮诱导不同的基因表达谱，然而，存活的移植口腔粘膜上皮的表型是相同的，在体内的口腔粘膜上皮表明，培养的口腔粘膜上皮不转分化为角膜上皮。这些基础研究结果有望为进一步了解眼表机制和开发新的治疗方法做出贡献。

项目成果

LRIG1 as a potential novel marker for neoplastic transformation in ocular surface squamous neoplasia.

• DOI: 10.1371/journal.pone.0093164
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Nagata M;Nakamura T;Sotozono C;Inatomi T;Yokoi N;Kinoshita S
• 通讯作者: Kinoshita S

Expression of prostaglandin E receptor subtype EP4 in conjunctival epithelium of patients with ocular surface disorders: case-control study.

• DOI: 10.1136/bmjopen-2012-001330
• 发表时间: 2012
• 期刊: BMJ open
• 影响因子: 2.9
• 作者: Ueta M;Sotozono C;Yamada K;Yokoi N;Inatomi T;Kinoshita S
• 通讯作者: Kinoshita S

Immunohistochemical analysis of inflammatory limbal conjunctiva adjacent to Mooren's ulcer

• DOI: 10.1136/bjophthalmol-2012-302631
• 发表时间: 2013-01
• 期刊: British Journal of Ophthalmology
• 影响因子: 4.1
• 作者: K. Shinomiya;M. Ueta;C. Sotozono;T. Inatomi;N. Yokoi;N. Koizumi;S. Kinoshita

Prostaglandin E receptor subtype EP3 expression in human conjunctival epithelium and its changes in various ocular surface disorders.

• DOI: 10.1371/journal.pone.0025209
• 发表时间: 2011
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Ueta M;Sotozono C;Yokoi N;Inatomi T;Kinoshita S
• 通讯作者: Kinoshita S

Cultivated Oral Mucosal Epithelial Transplantation for Ocular Surface Reconstruction

培养口腔粘膜上皮移植用于眼表重建

• 发表时间: 2013
• 作者: 慶野博;渡辺交世;中山真紀子;瀧和歌子;岡田アナベルあやめ;T Inatomi
• 通讯作者: T Inatomi