Gene Expression Profiling and de novo Transcriptome Sequencing using Geneballs

使用 Geneballs 进行基因表达谱分析和从头转录组测序

• 批准号: LP0349397
• 负责人: Prof David Hume
• 金额: $ 19.28万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-10-21 至 2006-10-20
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP0349397
• 关键词: Gene; Expression; Profiling; de; novo

The purpose of the project is to demonstrate that bead-based technology can be used in applications that currently require DNA hybridisation in order to overcome existing deficiencies in microarray technology. By providing the capability to quickly and efficiently produce, screen and utilize biomolecule libraries of nearly unlimited size, this technology provides the key to unlock the power of genomics and proteomics for use in real world applications. The project has two aspects. First, relatively small directed cDNA-bead libraries will be compared to known low-density cDNA microarrays to validate the technique for utility in gene expression profiling. Secondly, large libraries containing short oligonucleotide sequences will be used for de novo sequencing of a complete transcriptome. Proof-of-concept in either case will pave the way for many genomic applications and catapult the technology to 'blockbuster' status.

该项目的目的是证明，基于珠子的技术可以用于目前需要DNA杂交的应用，以克服微阵列技术中现有的不足。通过提供快速高效地生产、筛选和利用几乎无限大的生物分子文库的能力，这项技术提供了释放基因组学和蛋白质组学的力量以用于现实世界应用的钥匙。该项目有两个方面。首先，将相对较小的定向cdna-bead文库与已知的低密度cDNA微阵列进行比较，以验证该技术在基因表达谱中的实用性。其次，包含短寡核苷酸序列的大文库将用于完整转录组的从头测序。无论是哪种情况，概念验证都将为许多基因组应用铺平道路，并将这项技术推向“轰动一时”的地位。