Development of novel method of therapy for Vibrio vulnificus infeotion unlque to surrounding area of Ariak Sea

寻找一种感染创伤弧菌的噬菌体，并从有明海沉积物中分离出4种噬菌体。所有分离的噬菌体仅能感染创伤弧菌NBRC 15645^T，这导致宿主特异性很窄。电镜分析显示噬菌体1号的大小为200 nm×800 nm。研究了分离噬菌体感染创伤弧菌NBRC 15645^T的裂解曲线。此外，从分离的噬菌体中提取基因组，但产量相当低。另一方面，我们在C57BL/J6小鼠后腿底部皮下注射创伤弧菌ATCC27562^T株或创伤弧菌K-6株，两种创伤弧菌感染的动物模型均成功。此外，我们还在6只C57BL/J6小鼠的后腿底部皮下注射经其噬菌体溶原的K-6菌株或L-K-6菌株。结果，注射K-6毒株后，6只小鼠中有4只死亡，而注射L-K-6毒株后，没有小鼠死亡。与K-6菌株相比，L-K-6菌株的生长速率降低，溶血活性提高。

A bacteriophage which infects Vibrio vulnificus was searched, and four bacteriophages were isolated from the sediments in Ariake Sea. All isolated bacteriophages could infect only V.vulnificus NBRC 15645^T, which resulted in a quite narrow host specificity. Electron microscopical analysis revealed that the size of bacteriophage No.1 was 200 nm×800 nm. The lysis curve of isolated bacteriophages infecting V.vulnificus NBRC 15645^T were also investigated. Furthermore, a genome extraction from isolated bacteriophages was performed, but the yields were quite low. On the other hand, we gave a hypodermic injection of V.vulnificus ATCC27562^T strain or V. vulnificus K-6 strain to C57BL/J6 mouse's hind leg at the bottom, which succeeded in animal model infected with both V.vulnificus strains. In addition, we also gave a hypodermic injection of K-6 strain or L-K-6 strain, which was lysogenized with its bacteriophage, to six C57BL/J6 mice's hind leg at the bottom. As a result, the injection of K-6 strain killed four of six mice while no mice were killed by L-K-6 strain. Furthermore, L-K-6 strain decreased the growth rate and increase hemolytic activity, compared with K-6 strain.