Development of novel method of therapy for Vibrio vulnificus infeotion unlque to surrounding area of Ariak Sea

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A bacteriophage which infects Vibrio vulnificus was searched, and four bacteriophages were isolated from the sediments in Ariake Sea. All isolated bacteriophages could infect only V.vulnificus NBRC 15645^T, which resulted in a quite narrow host specificity. Electron microscopical analysis revealed that the size of bacteriophage No.1 was 200 nm×800 nm. The lysis curve of isolated bacteriophages infecting V.vulnificus NBRC 15645^T were also investigated. Furthermore, a genome extraction from isolated bacteriophages was performed, but the yields were quite low. On the other hand, we gave a hypodermic injection of V.vulnificus ATCC27562^T strain or V. vulnificus K-6 strain to C57BL/J6 mouse's hind leg at the bottom, which succeeded in animal model infected with both V.vulnificus strains. In addition, we also gave a hypodermic injection of K-6 strain or L-K-6 strain, which was lysogenized with its bacteriophage, to six C57BL/J6 mice's hind leg at the bottom. As a result, the injection of K-6 strain killed four of six mice while no mice were killed by L-K-6 strain. Furthermore, L-K-6 strain decreased the growth rate and increase hemolytic activity, compared with K-6 strain.