Development of analysis system of gene expression cascade using in vitro virus

由于顺式调控DNA元件与转录因子之间的特异性相互作用是转录调控网络的关键组成部分，因此，对DNA-转录因子相互作用的全面分析将有助于深入了解细胞增殖、组织形态发生和疾病的发育过程。在这个研究项目中，我第一次展示了体外病毒(IVV)选择系统，一种mRNA展示技术，可以用于阐明新的、具有生物学意义的DNA-蛋白质相互作用。这里描述的IVV系统将有助于大规模分析DNA-转录因子相互作用，以便在基因组范围内绘制转录调控网络的图谱。

Since the specific interactions between cis-regulatory DNA elements and transcription factors are critical components of transcriptional regulatory networks, comprehensive analysis of DNA-transcription factor interactions is expected to provide a deep understanding of the mechanisms of cell proliferation, developmental processes in tissue morphogenesis, and disease. In this research program, I have shown for the first time that in vitro virus (IVV) selection system, an mRNA display technique, can be employed for the elucidation of new, biologically significant DNA-protein interactions. The IVV system described herein will facilitate the large-scale analysis of DNA-transcription factor interactions for mapping of transcriptional regulatory networks on a genome-wide scale.