Basic research for improvement of productivity of bio-medicines based on cell quality control system

我们建立了用全内反射荧光显微镜分析内质网中货物蛋白迁移率的方法。为了处理大量的数据，我们提出了利用图像分析的方法。利用改进的时间和空间分辨率，我们现在能够分析细胞质蛋白质的简单扩散。最后，通过建立的方法确定温度胁迫是影响货物蛋白质流动性的因素之一。

We established the method for mobility analyses of cargo proteins in endoplasmic reticulam by total internal reflection fluorescence microscopy. To deal with the large amount of data, we proposed the method using image analyses. Using improved resolution in time and space, we are now able to analyze simple diffusion of cytoplasmic proteins. Finally, we identified temperature stress as one of the factors affected on cargo protein mobility by established methods.