Exproitation of new method to control ligninolytic enzymes production

控制木质素分解酶生产新方法的开发

• 批准号: 21580404
• 负责人: IRIE Toshikazu
• 金额: $ 3.16万
• 依托单位: The University of Shiga Prefecture
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21580404/
• 关键词: 白色腐朽菌; カルモデュリン; 担子菌門; リグニン分解酵素; きのこ; 担子菌; カルシウムシグナリング; cAMP; 自色腐朽菌

White-rot fungi are known to have a powerful ligninolytic system that can completely degrade wood lignin as well as persistent organic pollutants such as dioxin. This ability may be applicable to the construction of a novel potent bioreactor system to convert wood to potent materials and energy sources with low environmental load and to bioremediate polluted environments. However, the ligninolytic property of these fungi is attributable to many known and unknown enzyme genes, expression of which is inductive, and the factors that determine this expression are not completely understood. The lack of knowledge regarding the ligninolytic property of these fungi is an impediment to the development of a highly effective lignin-degrading fungal strain for the construction of an efficient bioreactor system. The identification of a master regulator that regulates the entire ligninolytic system in white-rot fungi could be used as a target for breeding a high lignin-degrading strain and for furth … More ering our understanding of the lignin-degradation system in these fungi.In this study, we conducted a transcriptome analysis during the early development of Lignin peroxidase(LiP) and manganese peroxidase(MnP) in Phanerochaete chrysosporium, which is one of the most widely researched white-rot fungus in the world. LiP and MnP are typical ligninolytic enzymes and considered good indicators for ligninolytic system expression. The transcriptome analysis indicated that the gene encoding calmodulin(CaM) was expressed in parallel with the lip and mnp genes. Then, we investigated effects of a calmodulin(CaM) inhibitor, W-7 on the expression of LiP and MnP genes in P. chrysosporium in order to consider the role of CaM. As a result, it was suggest that CaM has an important role for the expression of isozyme genes of LiP and MnP at the transcription level and that cAMP signaling functions to increase the transcription of LiP and MnP through the induction of cam transcription. We are currently investigating CaM-interacting proteins to analyze the downstream pathway regulated by CaM with the aim to identify a breeding target for construction of novel high lignin-degrading strains. Less

白腐真菌具有强大的木质素降解系统，可以完全降解木材木质素以及二恶英等持久性有机污染物。这种能力可能适用于构建一种新的有效的生物反应器系统，以将木材转化为具有低环境负荷的有效材料和能源，并对污染的环境进行生物修复。然而，这些真菌的木质素分解特性可归因于许多已知和未知的酶基因，其表达是诱导性的，并且决定这种表达的因素尚未完全了解。缺乏关于这些真菌的木质素降解性质的知识是开发用于构建有效生物反应器系统的高效木质素降解真菌菌株的障碍。白腐真菌木质素降解系统的主要调控因子的鉴定可作为选育高效木质素降解菌株的目标，并可进一步研究木质素降解的机理。 ...更多信息 本研究以白腐真菌黄孢原毛平革菌为研究对象，对黄孢原毛平革菌木质素过氧化物酶（LiP）和锰过氧化物酶（MNP）的早期发育过程进行了转录组学分析。LiP和MnP是典型的木质素降解酶，被认为是木质素降解系统表达的良好指示剂。转录组分析表明，编码钙调素（CaM）的基因与lip和mnp基因平行表达。然后，我们研究了钙调素（CaM）抑制剂W-7对黄孢原毛平革菌中LiP和MNP基因表达的影响，以考虑CaM的作用。结果表明，CaM在转录水平上对LiP和MNP同工酶基因的表达起重要作用，cAMP信号通过诱导CaM的转录而促进LiP和MNP的转录。我们目前正在研究CaM相互作用蛋白，以分析CaM调控的下游途径，目的是确定一个育种目标，构建新型的高木质素降解菌株。少

项目成果

白色腐朽菌におけるリグニン分解系発現調節遺伝子の解析

白腐真菌木质素分解系统表达调控基因分析

• 发表时间: 2009
• 作者: 高橋美佐;坂本敦;江面浩;森川弘道;Sato Y;入江俊一
• 通讯作者: 入江俊一

Transcriptional effect of a calmodulin inhibitor, W-7, on the ligninolytic enzyme genes in Phanerochaete chrysosporium

• DOI: 10.1007/s00294-010-0309-z
• 发表时间: 2010-10-01
• 期刊: CURRENT GENETICS
• 影响因子: 2.5
• 作者: Sakamoto, Takaiku;Kitaura, Hironori;Irie, Toshikazu
• 通讯作者: Irie, Toshikazu

Changes in the gene expression of the white rot fungus Phanerochaete chrysosporium by the addition of atropine

添加阿托品对白腐菌黄孢原毛平革菌基因表达的影响

• 发表时间: 2009
• 期刊: Bioscience, Biotechnology and Biochemistry
• 作者: Minami M.;K. Suzuki;A. Shimizu;T. Hongo;T. Sakamoto;N. Ohyama;H. Kitaura;A. Kusaka;K. Iwama;and T. Irie
• 通讯作者: and T. Irie

Effects of cAMP on ligninolytic enzymes production in Phanerochaete chrysosporium

cAMP对黄孢原毛平革菌木质素分解酶产生的影响

• 发表时间: 2011
• 作者: Yao Y.;T. Sakamoto;Y. Honda;T. Watanabe;W. Hashigaya;Y. Hida;K. Suzuki;T. Irie
• 通讯作者: T. Irie

リグニン分解酵素発現におけるcAMPシグナルの作用

cAMP信号对木质素降解酶表达的影响

• 发表时间: 2011
• 作者: 矢尾祐樹;阪本鷹行;橋ヶ谷渉;肥田嘉文;鈴木一実;本田与一、渡辺隆司;入江俊一
• 通讯作者: 入江俊一