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Utility of separation technique of live cancer stem cells with using an energy transfer fluorescence probes

Utility of separation technique of live cancer stem cells with using an energy transfer fluorescence probes
使用能量转移荧光探针分离活体癌症干细胞技术的实用性
批准号:
21590622
负责人:
TAKESHITA Akihiro
金额:
$3.0万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2011

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中文摘要
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英文摘要
QUAL/FRET method is effective to determine the cells in which have specific mRNA. In this study, we examined the efficacy on insertion of probes using fluorescein labeled dextran firstly. Relatively high molecular ones did not enter into nucleus and distributed in cytoplasm. On the other hand, the efficacy of the insertion was studied in cell lines and peripheral blood cells. The efficacy was not sufficient in adherent cells and lymphocytes. It should be improved by changing molecular size of probe and conjugated fluorescence.
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会议论文
Impact of additional chromosomal abnormalities(ACAs) in patients of acute promyelocytic leukemia10years follow up results of the Japan Adult Leukemia Study Group APL97study
日本成人白血病研究组APL97研究的额外染色体异常(ACAs)对急性早幼粒细胞白血病患者10年随访结果的影响
DOI: --
发表时间: 2010
期刊:
影响因子: --
作者: [Ono T, Takeshita A, Kishimoto Y, Kiyoi H, Okada M, Kawai Y, Tsuzuki M, Horikawa K, Matsuda M, Shinagawa K, Monma F, Ohtake S, Nakaseko C, Takahashi M, Yagasaki F, Kimura Y, Iwanaga M, Asou N, Naoe T, Ohno R]
通讯作者: Ohno R
DOI: --
发表时间: 2009
期刊:
影响因子: --
作者: [Watanabe H, Takeshita A, Oshida M, Fujihara H, Tomoda Y, Yurugi K Ohto H]
通讯作者: Yurugi K Ohto H
A Phase I/II Trial of Gemtuzumab Ozogamicin in Combination with Cytarabine and Anthracyclines(Daunorubicin or Idarubicin) for CD33-Positive Refractory or Relapsed AML Patients Younger Than65Years. Results of Phase I Parts of the JALSG-AML206Study
Gemtuzumab Ozogamicin 联合阿糖胞苷和蒽环类药物(柔红霉素或伊达比星)治疗 65 岁以下 CD33 阳性难治性或复发性 AML 患者的 I/II 期试验。
DOI: --
发表时间: 2009
期刊:
影响因子: --
作者: [Usui N, Takeshita A, Nakaseko C, Dobashi N, Fujita H, Kiyoi H, Kobayashi Y, Fujisawa S, Yagasaki F, Nishii K, Kiguchi T, Miyazaki Y, Ohtake S, Miyawaki S, Ohno R, Naoe T, Ohnishi K]
通讯作者: Ohnishi K
CMC-544(inotuzumab ozogamicin)shows less effect on multidrug resistant cells : analyses in cell lines and cells from patients with B-cell chronic lymphocytic leukaemia and lymphoma.
CMC-544(inotuzumab ozogamicin)对多重耐药细胞的影响较小:对 B 细胞慢性淋巴细胞白血病和淋巴瘤患者的细胞系和细胞进行分析。
DOI: --
发表时间: 2009
期刊: Br J Haemato 146
影响因子: --
作者: [Suzuki K, Nakase K, Kyo T, Katayama N, Akihiro Takeshita]
通讯作者: Akihiro Takeshita
36
    Isolation of cancer stem cells by the staining nucleic acid and analyses of their molecular specificities
    • 批准号:
      24590688
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.49万
    • 财政年份:
      2012
    • 负责人:
      TAKESHITA Akihiro
    • 依托单位:
    Identification of minimal residual cells after the treatment of leukemia cells by the imaging of mRNA
    • 批准号:
      19590552
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $2.91万
    • 财政年份:
      2007
    • 负责人:
      TAKESHITA Akihiro
    • 依托单位:
    Study of Resistance Mechanisms of All-trans Retinoic Acid in Acute Promyelocytic Leukemia
    • 批准号:
      10670939
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $1.92万
    • 财政年份:
      1998
    • 负责人:
      TAKESHITA Akihiro
    • 依托单位:
    海外基金