FGF signaling accelerate epithelial regeneration by Oral Epithelial Stem Cells

FGF 信号传导加速口腔上皮干细胞的上皮再生

• 批准号: 21592482
• 负责人: INOUE Masahiro
• 金额: $ 3万
• 依托单位: Osaka Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592482/
• 关键词: FGF; 口腔上皮; 幹細胞; 粘膜再生

Fibroblast growth factor-10(FGF-10) is a known epithelial mitogen in several organ systems, such as the lung, that acts in a bidirectional paracrine manner and acts exclusively through a subset of receptors such as FGFr2b. However, its role in oral epithelial regeneration is unknown. We investigated the role of FGFr2b/FGF-10 signaling in oral epithelial wound healing using FGF-10 overexpression(OE) and FGFr2b attenuation/knock-down(KD) transgenic mice. The present results suggested that, 1) Under-developing of the epithelial layer was noted in the FGFr2b KD mice, whereas over-developing was noted in the FGF-10 OE mice. 2) FGF10 overexpression initiated the parakeratosis of oral epithelium or urothelium and 3) FGF10 overexpression worked the proliferation of the basal laminae in the stratified squamous epithelium and transitional epithelium. Form these results, FGF-10/FGFr2b signaling appears to play an active proliferation in Oral Epithelial Stem Cells, and accelerate the regenerative capacity of the oral epithelium.

成纤维细胞生长因子-10（FGF-10）是已知的几种器官系统（例如肺）中的上皮促分裂原，其以双向旁分泌方式起作用，并且仅通过受体的子集（例如FGFr 2b）起作用。然而，其在口腔上皮再生中的作用尚不清楚。我们使用FGF-10过表达（OE）和FGFr 2b衰减/敲低（KD）转基因小鼠研究了FGFr 2b/FGF-10信号传导在口腔上皮伤口愈合中的作用。结果表明：（1）FGFr 2b KD小鼠上皮层发育不足，而FGF-10 OE小鼠上皮层发育过度。2)FGF 10过表达可引起口腔上皮或尿道上皮角化不全; 3）FGF 10过表达可引起复层鳞状上皮和移行上皮基底层增生。从这些结果来看，FGF-10/FGFr 2b信号通路似乎在口腔上皮干细胞中起着积极的增殖作用，并加速口腔上皮的再生能力。

项目成果

A Potential Role for FGF-10/FGFr2b Signaling in Bladder Homeostasis

FGF-10/FGFr2b 信号在膀胱稳态中的潜在作用

• 发表时间: 2010
• 作者: Kuremoto K;Yamzon J;Choi IS;Lee KH;Bellusci S;Warburton D;Koh K
• 通讯作者: Koh K

FGFR2b signaling and ameloblast stem cells in mouse incisor regeneration

FGFR2b 信号传导和成釉细胞干细胞在小鼠门牙再生中的作用

• 发表时间: 2010
• 作者: 呉本晃一;他
• 通讯作者: 他

FGFR2b signaling and ameloblast stem cells in mouse incisor regeneration.

FGFR2b 信号传导和成釉细胞干细胞在小鼠门牙再生中的作用。

• 发表时间: 2010
• 作者: Kuremoto K;他
• 通讯作者: 他

Fibroblast Growth Factor 10-mediated Delay in Bladder Wound Healing in Inducible Transgenic Mice

成纤维细胞生长因子 10 介导的诱导转基因小鼠膀胱伤口愈合延迟

• 发表时间: 2009
• 作者: Kuremoto K;他;伊藤秀高;Koh-ichi Kuremoto
• 通讯作者: Koh-ichi Kuremoto

歯の発生及びエナメル芽細胞分化におけるcore-binding factor β(Cbfb)の役割について

核心结合因子β（Cbfb）在牙齿发育和成釉细胞分化中的作用

• 发表时间: 2010
• 作者: 黒坂寛;早野暁;呉本晃一;原田英光;山城隆
• 通讯作者: 山城隆