The research for the niche factor of stem cells in culture condition

干细胞培养条件下的生态位因子研究

• 批准号: 21592584
• 负责人: KIKUIRI Takashi
• 金额: $ 3万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21592584/
• 关键词: 乳歯歯髄由来幹細胞; 未分化維持; 継代培養; Wntシグナル; 歯学; 歯根膜由来幹細胞; 分化

Stem cells from human exfoliated deciduous teeth(SHED) are stem cell that differentiate into osteocyte and adipocyte. In this study, we investigated whether the Wnt signaling inhibitor has a potency of the stemness of SHED after long-term cell culture or not. We demonstrated that SHED treated with the Wnt signaling inhibitor was capable of stemness in vitro experiment and generating bone structures at 8 weeks post-transplantation into nude mouse. These data suggested that the Wnt signaling inhibitor acts as a stemness factor on SHED after long-term cell culture

脱落型乳牙干细胞是一种可分化为骨细胞和脂肪细胞的干细胞。在这项研究中，我们研究了Wnt信号抑制剂是否具有长期培养细胞后脱落物的茎的效力。我们证明了Wnt信号抑制剂处理的SHEAD能够在裸鼠体内移植8周后进行体外干化实验和骨结构的形成。这些数据表明，Wnt信号抑制因子在长期细胞培养后的脱细胞中起到了茎的作用

项目成果

Platinum nanoparticles suppress osteoclastogenesis through scavenging of ROS productions in RAW 264. 7 cells

铂纳米颗粒通过清除 RAW 264. 7 细胞中产生的 ROS 来抑制破骨细胞生成

• 发表时间: 2011
• 期刊: Journal of Pharmacological Sciences
• 影响因子: 3.5
• 作者: Mayumi Nomura;Yoshitaka Yoshimura;Takashi Kikuiri;Tomokazu Hasegawa;Yumi Taniguchi;Yoshiaki Deyama;Ken-ichi Koshiro;Hidehiko Sano;Kuniaki Suzuki and Nobuo Inoue
• 通讯作者: Kuniaki Suzuki and Nobuo Inoue

Stage-dependent suppression of the formation of dentin-resorbing multinuclear cells with MIF in vitro

体外 MIF 对牙本质吸收多核细胞形成的阶段依赖性抑制

• DOI: 10.3892/etm.2011.362
• 发表时间: 2011
• 期刊: Experimental and Therapeutic Medicine
• 影响因子: 2.7
• 作者: Kikuiri T;Yoshimura Y;Tabata F;Hasegawa T;Nishihira J;Shirakawa T
• 通讯作者: Shirakawa T

間葉系幹細胞と造血系幹細胞の併用による骨組織再生の試み

尝试使用间充质干细胞和造血干细胞的组合来再生骨组织

• 发表时间: 2011
• 作者: 高崎千尋;大島昇平;岡田理恵子;八若保孝;松野美乃
• 通讯作者: 松野美乃

Effect of the release from mechanical stress on osteoclastogenesis in RAW264.7 cells

• DOI: 10.3892/ijmm.2011.675
• 发表时间: 2011-07-01
• 期刊: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
• 影响因子: 5.4
• 作者: Shibata, Kenjiro;Yoshimura, Yoshitaka;Iida, Junichiro
• 通讯作者: Iida, Junichiro

Platinum Nanoparticles Suppress Osteoclastogenesis Through Scavenging of Reactive Oxygen Species Produced in RAW264.7 Cells

• DOI: 10.1254/jphs.11099fp
• 发表时间: 2011-12-01
• 期刊: JOURNAL OF PHARMACOLOGICAL SCIENCES
• 影响因子: 3.5
• 作者: Nomura, Mayumi;Yoshimura, Yoshitaka;Inoue, Nobuo
• 通讯作者: Inoue, Nobuo