The research for the niche factor of stem cells in culture condition

脱落型乳牙干细胞是一种可分化为骨细胞和脂肪细胞的干细胞。在这项研究中，我们研究了Wnt信号抑制剂是否具有长期培养细胞后脱落物的茎的效力。我们证明了Wnt信号抑制剂处理的SHEAD能够在裸鼠体内移植8周后进行体外干化实验和骨结构的形成。这些数据表明，Wnt信号抑制因子在长期细胞培养后的脱细胞中起到了茎的作用

Stem cells from human exfoliated deciduous teeth(SHED) are stem cell that differentiate into osteocyte and adipocyte. In this study, we investigated whether the Wnt signaling inhibitor has a potency of the stemness of SHED after long-term cell culture or not. We demonstrated that SHED treated with the Wnt signaling inhibitor was capable of stemness in vitro experiment and generating bone structures at 8 weeks post-transplantation into nude mouse. These data suggested that the Wnt signaling inhibitor acts as a stemness factor on SHED after long-term cell culture