Analysis of SHIP related to osteoclastogenesis

含SH 2的肌醇-5 '-磷酸酶（SHIP）与免疫反应有关，对巨噬细胞集落刺激因子（M-CSF）和核因子κ B受体激活剂配体（RANKL）诱导的破骨细胞前体细胞增殖具有负性调节作用。在这项研究中，我们研究了SHIP对TNF-α诱导的破骨细胞生成的影响。用M-CSF和TNF-α培养SHIP+/+和SHIP-/-小鼠的骨髓细胞。与SHIP+/+细胞相比，SHIP-/-细胞中TRAP阳性细胞的数量显著增加。这些结果表明，SHIP可能负调控破骨细胞前体细胞的增殖。

SH2-containing inositol-5'-phosphatase(SHIP), which is related to immunoreaction, has been reported to negatively regulate the proliferation of osteoclast precursors induced by macrophage colony-stimulating factor(M-CSF) and ligand for receptor activator of nuclear factor kappa B(RANKL). In this study, we investigated the effects of SHIP on TNF-α-induced osteoclastogenesis. Bone marrow cells from SHIP+/+ and SHIP-/-mice were cultured with M-CSF and TNF-α. The number of TRAP-positive cells was significantly increased in SHIP-/-cells compared with SHIP+/+ cells. These results suggest that SHIP may negatively regulate the proliferation of osteoclast precursors.