Epigenetic regulation of embryonic and somatic stem cells by Polycomb

使用Ring1A/B条件基因敲除胚胎干细胞的分析表明，Ring1A/B通过抑制发育调节因子而有助于维持ES细胞的特性。我们还报道了Ring1A/B介导的组蛋白H2A的单一泛素化是转录抑制靶基因的必要步骤。此外，我们还发现Ring1A/B的组蛋白泛素化活性也是维持来自6.5DPC胚胎的外胚祖细胞所必需的。

Analysis using Ring1A/B conditional knockout embryonic stem(ES) cells revealed that Ring1A/B contribute to maintain ES cell identity by repressing developmental regulators. We also reported that Ring1A/B-mediated mono-ubiquitination of histone H2A is an essential step for transcriptional repression of the target genes. Furthermore, we found that histone ubiquitination activity of Ring1A/B is also required for the maintenance of epiblast stem cells derived from a 6.5 dpc embryo.