ANALYSIS OF DYNAMIC BEHAVIOR OF RNA USING LIGHT-UP RNA MODULE AS A TAG

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The fluorescence intensity of tobramycin-bearing phenyl-BODIPY as a probe increases upon interaction with tobramycin-aptamer, an RNA sequence that specifically binds to tobramycin. Transcription of a target gene (tRNA in this case) containing the aptamer sequence downstream thereof affords a fused (target-aptamer) RNA, the aptamer component of which can be detected by enhanced fluorescence, although no big, in the presence of the probe. In this way, the transcription of the target gene can be fluorescence monitored.