Structuresandfunctionsofthespermubiquitin-proteasomesystem involvedlnfertilizationandreproduction

参与受精和繁殖的精子泛素-蛋白酶体系统的结构和功能

• 批准号: 21390019
• 负责人: SAWADA Hitoshi
• 金额: $ 11.9万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390019/
• 关键词: 受精; ユビキチン; プロテアソーム; 精子; 卵; ホヤ; プロテアーゼ

We previously reported that the ubiquitin-proteasome system(UPS) plays a key extracellular role in fertilization, as an egg-coat lysin, which allows sperm to penetrate through the egg-coat in the ascidians H. roretziand C. intestinalis. However, the structural features and the sorting mechanism of the sperm UPS are not known. Here, we investigated the sperm-specific UPS using these ascidians. We found that a certain ubiquitin-conjugating enzyme(Ube2r) is specifically expressed in the testis of C. intestinalis and that the C-terminal 16 residues of theα6/PSMA1 subunit of the sperm 20S proteasome are specifically processed in H. roretzi. These findings enabled us to further investigate the possible involvement of the C-terminal processing of the proteasome in the sorting of the proteasome to an acrosome or outer surface of sperm head regions.

我们之前报道过，泛素蛋白酶体系统（UPS）在受精过程中发挥着关键的细胞外作用，作为一种卵衣溶素，它可以使海鞘中的精子穿透卵衣。然而，精子UPS的结构特征和分选机制尚不清楚。在这里，我们使用这些海鞘研究了精子特异性 UPS。我们发现某种泛素结合酶（Ube2r）在C. Enteris的睾丸中特异性表达，并且精子20S蛋白酶体的α6/PSMA1亚基的C端16个残基在H. roretzi中被特异性加工。这些发现使我们能够进一步研究蛋白酶体的 C 末端加工在蛋白酶体到顶体或精子头部区域外表面的分选中的可能参与。

项目成果

Identification of testis‐specific ubiquitin‐conjugating enzyme in the ascidian Ciona intestinalis

• DOI: 10.1002/mrd.21198
• 发表时间: 2010-07
• 期刊: Molecular Reproduction and Development
• 影响因子: 2.5
• 作者: Naoto Yokota;Y. Harada;H. Sawada

Comprehensive Egg Coat Proteome of the Ascidian Ciona intestinalis Reveals Gamete Recognition Molecules Involved in Self-sterility*

• DOI: 10.1074/jbc.m809672200
• 发表时间: 2009-04
• 期刊: Journal of Biological Chemistry
• 影响因子: 4.8
• 作者: Lixy Yamada;Takako Saito;H. Taniguchi;H. Sawada;Y. Harada

Ascidian sperm self/nonself-recognition and ubiquitin-proteasome systems

海鞘精子自我/非自我识别和泛素蛋白酶体系统

• 发表时间: 2011
• 作者: Hitoshi Sawada

Sperm-specific C-terminal processing of the proteasome PSMA1/α6 subunit

蛋白酶体 PSMA1/α6 亚基的精子特异性 C 末端加工

• 发表时间: 2011
• 期刊: Biochem. Biophys. Res. Commun
• 作者: Naoto Yokota;Yohei Kataoka;Noritaka Hashii;Nana Kawasaki;and Hitoshi Sawada.
• 通讯作者: and Hitoshi Sawada.

カタユウレイボヤにおける精子膜タンパク質のプロテオーム解析

海鞘精子膜蛋白的蛋白质组学分析

• 发表时间: 2010
• 作者: 横田直人;磯見拓也;谷口寿章;山田力志;澤田均
• 通讯作者: 澤田均