Analysis of pathogenesis of refractory childhood myelodysplastic syndrome using disease-specific iPS cells

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The cultured CD34-positive cells of juvenile myelomonocytic leukemia(JMML) patient were transfected the Yamanaka 4 genes, and cultured on mouse embryonic fibroblasts. Two-3 weeks later, many cell clusters with the RAS mutation were formed and negative for CD34. These cells were compatible with induced pluripotent(iPS) stem cells. We succeeded the establishment of iPS cells with myeloid differentiation potencial from the cultured JMML CD34-positive cells. We are going to elucidate pathogenesis of JMML and properties of the leukemic stem cells.