Optical analysis of functional development of the spinal cord neural circuits

脊髓神经回路功能发育的光学分析

• 批准号: 21500368
• 负责人: SATO Katsushige
• 金额: $ 2.83万
• 依托单位: Komazawa Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21500368/
• 关键词: 光学イメージング; 膜電位感受性色素; 中枢神経系; 機能発生; embryo; 脊髄; 光学計測; 鶏胚; 生体染色; 脊髄神経

Spontaneous correlated neuronal activity during early development spreads like a wave by recruiting a large number of neurons and is considered to play a fundamental role in neural development. One important and yet unresolved question is where the activity originates, especially at the earliest stage of wave expression. In other words, which part of the brain differentiates first as a source of the correlated activity, and how does it change as development proceeds. We assessed this issue by examining the spatiotemporal patterns of the depolarization wave, the optically-identified primordial correlated activity, using the optical imaging technique with voltage-sensitive dyes. We surveyed the region responsible for the induction of the evoked and spontaneous depolarization waves in chick embryos and traced its developmental changes. The results showed that the wave initially originated in a restricted area near the obex and was generated by multiple regions at later stages. We suggest that the upper cervical cord/lower medulla near the obex is the kernel which differentiates first as the source of the correlated activity, and that regional and temporal differences in neuronal excitability might underlie the developmental profile of wave generation in early chick embryos.

早期发育期间自发相关的神经元活动像波浪一样通过招募大量神经元，被认为在神经发育中起着基本作用。一个重要但尚未解决的问题是活动的起源，尤其是在波浪表达的最早阶段。换句话说，大脑的哪一部分首先将其作为相关活动的来源，以及随着发展的进行如何变化。我们使用具有电压敏感染料的光学成像技术检查去极化波的时空模式，即光学识别的原始相关活性，从而评估了这个问题。我们调查了负责诱导鸡胚胎中诱发和自发去极化波的区域，并追溯了其发育变化。结果表明，该波最初起源于OBEX附近的受限区域，并在后期阶段由多个区域生成。我们建议，OBEX附近的上颈绳/下髓质是首先将其区分为相关活性来源的内核，而神经元兴奋性的区域和时间差异可能是早期雏鸡胚胎中波产生的发展谱的基础。

项目成果

The embryonic brain and development of vagal pathways

• DOI: 10.1016/j.resp.2011.01.012
• 发表时间: 2011-08-31
• 期刊: RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
• 影响因子: 2.3
• 作者: Momose-Sato, Yoko;Sato, Katsushige
• 通讯作者: Sato, Katsushige

NR2B-dependent neural activation is indispensable for visual imprinting

NR2B依赖性神经激活对于视觉印记是不可或缺的

• 发表时间: 2011
• 作者: Tomoharu Nakamori;Katsushige Sato;Kohichi Tanaka;Hiroko Ohki-Hamazaki
• 通讯作者: Hiroko Ohki-Hamazaki

Monitoring population membrane potential signals during functional development of neuronal circuits in vertebrate embryos. In : Membrane Potential Imaging in the Nervous System : Methods and Applications

监测脊椎动物胚胎神经元回路功能发育过程中的群体膜电位信号。

• 发表时间: 2010
• 作者: Momose-Sato;Y.;Sato;K. and Kamino;K.
• 通讯作者: K.

Spreading synchronized activity in the embryonic brainstem and spinal cord. In : Comprehensive Developmental Neuroscience

在胚胎脑干和脊髓中传播同步活动。

• 作者: Momose-Sato;Y. and Sato;K.
• 通讯作者: K.

Spontaneous depolarization wave in the mouse embryo : spatial segregation with development and its mechanism

小鼠胚胎自发去极化波：发育过程中的空间分离及其机制

• 发表时间: 2011
• 作者: Momose-Sato;Y. and Sato;K
• 通讯作者: K