Development of ex vivo expansion system of hematopoietic stem/progenitor cells using stromal cells fixed on three-dimensional scaffolds

研究了使用固定在三维支架上的基质细胞体外扩增造血干细胞以将该方法应用于造血干细胞移植。本研究首先将基质细胞培养在三维多孔支架上，然后用有机溶剂（丙酮、甲醇或乙醇）固定。通过在固定的基质细胞上培养造血细胞来进行造血细胞的扩增，并检查有机溶剂对造血细胞扩增的影响。从这些培养实验中，证明了当基质细胞用丙酮固定时，造血细胞的扩增达到最高，并且用乙醇（毒性较小的溶剂）固定的基质细胞也有效地扩增造血细胞。

Ex vivo expansion of hematopoietic stem cells using stromal cells that were fixed on three-dimensional scaffolds was investigated to apply this method to hematopoietic stem cell transplantation. In this study, stromal cells were firstly cultured on the three-dimensional porous scaffolds, and then fixed by organic solvent (acetone, methanol or ethanol). Expansion of hematopoietic cells was performed by cultivating them on the fixed stromal cells, and effects of the organic solvents on the hematopoietic cell expansion were examined. From these culture experiments, it was demonstrated that highest expansion of the hematopoietic cells was achieved when the stromal cells were fixed by acetone, and that the stromal cells fixed by ethanol, less toxic solvent, were also effective in expanding the hematopoietic cells.