Development of ex vivo expansion system of hematopoietic stem/progenitor cells using stromal cells fixed on three-dimensional scaffolds

使用固定在三维支架上的基质细胞开发造血干/祖细胞的离体扩增系统

• 批准号: 21500422
• 负责人: MIYOSHI Hirotoshi
• 金额: $ 2.91万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21500422/
• 关键词: 造血幹細胞; ストローマ細胞; 胎仔肝臓細胞; 固定; 凍結保存; 分化・増殖; 三次元培養; ティッシュ・エンジニアリング

Ex vivo expansion of hematopoietic stem cells using stromal cells that were fixed on three-dimensional scaffolds was investigated to apply this method to hematopoietic stem cell transplantation. In this study, stromal cells were firstly cultured on the three-dimensional porous scaffolds, and then fixed by organic solvent (acetone, methanol or ethanol). Expansion of hematopoietic cells was performed by cultivating them on the fixed stromal cells, and effects of the organic solvents on the hematopoietic cell expansion were examined. From these culture experiments, it was demonstrated that highest expansion of the hematopoietic cells was achieved when the stromal cells were fixed by acetone, and that the stromal cells fixed by ethanol, less toxic solvent, were also effective in expanding the hematopoietic cells.

研究了将基质细胞固定在三维支架上体外扩增造血干细胞，并将该方法应用于造血干细胞移植。本研究首先在三维多孔支架上培养基质细胞，然后用有机溶剂（丙酮、甲醇或乙醇）固定基质细胞。在固定基质细胞上培养造血细胞，观察有机溶剂对造血细胞扩增的影响。这些培养实验表明，用丙酮固定基质细胞时，造血细胞的扩增率最高，用毒性较小的溶剂乙醇固定基质细胞也能有效地扩增造血细胞。

项目成果

皆川和則

皆川和则

• 发表时间: 2009
• 作者: 三好浩稔;小山寿恵;大根田修
• 通讯作者: 大根田修

Cryopreservation of fibroblasts immobilized within a porous scaffold : effects of preculture and collagen coating of scaffold on performance of three-dimensional cryopreservation

多孔支架内固定的成纤维细胞的冷冻保存：预培养和支架胶原涂层对三维冷冻保存性能的影响

• 发表时间: 2010
• 期刊: Artif Organs
• 影响因子: 2.4
• 作者: Miyoshi H;Ehashi T;Ohshima N;Jagawa A
• 通讯作者: Jagawa A

血管内皮細胞株との三次元共培養がマウス胎仔肝臓細胞の増殖・分化に及ぼす影響

与血管内皮细胞系三维共培养对小鼠胎肝细胞增殖和分化的影响

• 发表时间: 2009
• 作者: 三好浩稔;小山寿恵;大根田修;皆川和則
• 通讯作者: 皆川和則

Three-dimensional perfusion culture of mouse bone marrow cells within a porous polymer scaffold : effects of oxygen concentrationand stromal layer on expansion of haematopoietic progenitor cells

多孔聚合物支架内小鼠骨髓细胞的三维灌注培养：氧浓度和基质层对造血祖细胞扩增的影响

• 发表时间: 2011
• 期刊: Tissue Eng Regen Med
• 影响因子: 3.6
• 作者: Miyoshi H;Murao M;Ohshima N;TunT
• 通讯作者: TunT

Three‐dimensional culture of mouse bone marrow cells within a porous polymer scaffold: effects of oxygen concentration and stromal layer on expansion of haematopoietic progenitor cells

• DOI: 10.1002/term.295
• 发表时间: 2011-02
• 期刊: Journal of Tissue Engineering and Regenerative Medicine
• 影响因子: 3.3
• 作者: Hirotoshi Miyoshi;M. Murao;N. Ohshima;T. Tun