Analysis of renal function with isolated pronephros of Xenopus frog.

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To understand integrative renal functions in vertebrates, we explored and characterized genes expressing in pronephros of amphibian tadpole and mesonephros of teleost. We constructed the GFP expression vector linked 5'-upstream promoter of Na^+, K^+-ATPase, marker protein of renal tubule, and succeeded in isolating a living pronephric tubule by using transgenic technique. Furthermore, we explored genes associated with polycystic kidney disease (PKD) by a PCR-based subtractive hybridization method using pc mutant, a PKD model of medaka. As a result, we identified 2 genes (vasotocin type-2 receptor and aquaporin-8) reduced markedly in the kidney of pc mutant. Currently, we explore genes expressing in pronephros by global analysis and then characterize 2 genes found as candidate gene associated with teleost PKD.