Structure-function analysis of budding yeast cohesin complex involved in sister chromatid cohesion

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Cohesin complex is involved in holding replicated sister chromatids. Sister chromatid cohesion is conserved among eukaryotes and is required for faithful segregation of chromosomes. Cohesin complex is comprised of Smc proteins and non-Smc proteins. However, the structure-function relationship of this complex remains elusive. We have used high speed atomic force microscopy to visualize the cohesin complex. We could clearly see ATPase domain, coiled coil region and dimerization domain. We found that this domain organization is conserved among other Smc complexes and the stability of coiled coil region was observed.