Genome-wide screening of essential genes showing haplo-insufficiency in secretory protein production in yeast
Genome-wide screening of essential genes showing haplo-insufficiency in secretory protein production in yeast
批准号:
21780076
负责人:
HOSHIDA Hisashi
金额:
$2.75万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2011
中文摘要
为了鉴定影响酿酒酵母外源基因表达的基因,采用杂合敲除菌株对必要基因进行敲除。结果,鉴定出7个增强分泌型荧光素酶表达的突变体。另一方面,除弱生长突变体外,除弱生长突变体外,所有敲除菌株的表达均未显著降低。敲除菌株中有6个基因参与RNA代谢。
英文摘要
To identify the genes affecting heterologous gene expression in Saccharomyces cerevisiae, heterozygous knockout strains of essential genes were used. As a result, 7 mutants that enhance expression of a secretory luciferase were identified. On the other hand none of knockout strain showed significantly lower expression except for weak-growth mutants. Six genes among the knockout strains were involved in RNA metabolism.
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科研奖励(0)
会议论文
酵母Saccharomyces cerevisiae snt309破壊株での組換えタンパク質高発現
重组蛋白在酿酒酵母snt309破坏菌株中高表达
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[小林隆文, 矢村寿啓, 星田尚司, 赤田倫治]
通讯作者:
赤田倫治
開始コドン上流とORF中の配列が遺伝子発現に与える影響
起始密码子上游和 ORF 内的序列对基因表达的影响
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[平川雄基, 星田尚司, 赤田倫治]
通讯作者:
赤田倫治
Analysis of enhanced protein production mediated by 5'-UTR intron
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批准号:17K08198
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.91万
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财政年份:2017
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负责人:HOSHIDA Hisashi
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依托单位:
海外基金