Search for novel families of terpene cyclases from bacteria and analyses of physiological functions and bioactivities of C35 terpenes
Search for novel families of terpene cyclases from bacteria and analyses of physiological functions and bioactivities of C35 terpenes
批准号:
21780109
负责人:
SATO Tsutomu
金额:
$2.91万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2009
资助国家:
日本
项目状态:
已结题
起止时间:
2009 至 2010
中文摘要
通过对分枝杆菌C_ 35 萜烯的研究,发现了优先合成C_ 35 或C_ 50 产物的双功能Z-异戊二烯基转移酶。两种新的C_ 35 萜烯的结构表明,还原E和Z异戊二烯基残基的异戊烯基还原酶负责C_ 35 萜烯的生物合成。另一方面,来自枯草芽孢杆菌的萜烯环化酶和四异戊二烯基-β-姜黄烯环化酶的新家族可以通过体外酶促反应来鉴定。
英文摘要
A bifunctional Z-prenyltransferase that preferentially synthesize C_<35> or C_<50> products was found from the study on the mycobacterial C_<35> terpenes. The structure of two novel C_<35> terpenes suggested that a prenyl reductase reducing both E and Z prenyl residues was responsible for the biosynthesis of C_<35> terpenes. On the other hand, a novel family of terpene cyclases and tetraprenyl-β-curcumene cyclase from Bacillus subtilis could be identified by the in vitro enzymatic reaction.
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Mycobacterium tuberculosis H37Rv3378c 酵素の再検討
重新审视结核分枝杆菌 H37Rv3378c 酶
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[大塚崇弘]
通讯作者:
大塚崇弘
枯草菌由来C_35テルペンの生合成:新型テルペン環化酵素のテトラプレニル-β-クルクメン合成酵素の同定
枯草芽孢杆菌C_35萜烯的生物合成:新型萜烯环化酶四异戊烯基-β-姜黄烯合酶的鉴定
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[石田快, 丹羽悠介, 小野奈津子, 山篠貴史, 水野猛, 猿渡隆佳, 吉田悟]
通讯作者:
吉田悟
豚肝ラノステロール合成酵素:オキシドスクアレン19 位のバルクサイズが環化反応に及ぼす影響
猪肝羊毛甾醇合酶:氧化角鲨烯19体积大小对环化反应的影响
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--
发表时间:
2011
期刊:
影响因子:
--
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[山篠貴史, 水野猛, 阿部奈緒美]
通讯作者:
阿部奈緒美
Novel compounds of octahydroheptaprenylmycolic acyl ester and monocyclic C35-terpene,heptaprenylcycline B, from nonpathogenicMycobacterium species.
来自非致病性分枝杆菌属的八氢庚烯基分枝菌酰酯和单环 C35-萜烯庚烯环素 B 的新型化合物。
DOI:
--
发表时间:
2010
期刊:
Biosci.Biotechnol.Biochem. 74
影响因子:
--
作者:
[Tsutomu SATO, Ryosuke TAKAGI, YurikoORITO, Eri ONO, Tsutomu HOSHINO]
通讯作者:
Tsutomu HOSHINO
非病原性マイコバクテリアに存在するユニークなC_35テルペンの生合成:Z型プレニルトランスフェラーゼの機能解析とデヒドロヘプタプレニルサイクリンの同定
非致病性分枝杆菌中独特的 C_35 萜的生物合成:Z 型异戊二烯基转移酶的功能分析和脱氢庚烯基环素的鉴定
DOI:
--
发表时间:
2010
期刊:
影响因子:
--
作者:
[渡辺賢二, 吉田悟, 星野紘子, 渡辺賢二, 渡辺賢二, 阿部奈緒美, 猿渡隆佳, 山口雄生, 佐藤努]
通讯作者:
佐藤努
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