Estimation of the absorption and metabolic mechanism of monoacylglycerols in the small intestine

单酰甘油在小肠中的吸收和代谢机制的估计

• 批准号: 21780127
• 负责人: MUROTA Kaeko
• 金额: $ 2.91万
• 依托单位: Kinki University (2010)The University of Tokushima (2009)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21780127/
• 关键词: 脂質; 脂肪酸結合タンパク質; モノグリセリド; 小腸

In this study, in vitro binding assay, cell culture study, and in vivo rat study were performed. In in vitro study, the binding abilities of monoacylglycerols (MGs) and free fatty acids to intestinal fatty-acid binding proteins (FABPs) were examined. The result has indicated that the carbon chain length of free fatty acids is a key factor to determine the binding ability, whereas MGs' binding was not clear. In the cell culture study, the stable Caco-2 cell line expressing CD36 membrane protein was established. Furthermore, the lymphatic transport of various dietary lipids was estimated using thoracic-lymph cannulated rats.

在本研究中，进行了体外结合试验、细胞培养研究和大鼠体内研究。在体外研究中，检测了单酰基甘油（MG）和游离脂肪酸与肠脂肪酸结合蛋白（FABPs）的结合能力。结果表明，游离脂肪酸的碳链长度是决定其结合能力的关键因素，而MG的结合作用不明显。在细胞培养研究中，建立了稳定表达CD 36膜蛋白的Caco-2细胞系。此外，各种饮食脂质的淋巴转运估计使用胸淋巴管大鼠。