Functional analysis of canonical and alternative NF-κB pathways in mature B lymphocyte differentiation.

核因子-κB途径主要通过两种途径被激活，即规范途径和替代途径。基因打靶研究表明，规范途径和替代途径对于成熟B细胞的发育都是必不可少的。然而，人们对这些途径如何控制成熟B细胞的发育知之甚少。我建立了p52NF-κB2的条件性敲入转基因，并与NEMO缺陷小鼠杂交。P52NF-κB2的表达挽救了NEMO缺乏的滤泡和边缘区B细胞的发育，但不能挽救B1细胞的发育。这一结果表明，规范的和交替的NF-κB途径控制静息卵泡中的共同靶基因，并且规范的NF-kB途径本身对B1细胞的发育是必不可少的。

NF-κB pathway is activated mainly in two ways, canonical and alternative pathways. Gene-targeting studies show that both canonical and alternative pathways are essential for mature B cell development. However it is a little known how these pathways controls the development of mature B cells. I established conditional knock-in transgenes of p52NF-κB2 and crossed with NEMO deficient mice. The expression of p52NF-κB2 rescues the development of NEMO deficient follicular and marginal zone B cells but not B1 cells. This result shows that canonical and alternative NF-κB pathways control common target genes in resting follicular and canonical NF-kB pathway in itself is essential for the development of B1 cells.