The analyses of mechanism to induce pemphigus using desmoglein 3-GFP transgenic mice

桥粒芯糖蛋白3-GFP转基因小鼠诱导天疱疮的机制分析

• 批准号: 21791096
• 负责人: YOSHIDA Kazue
• 金额: $ 2.83万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21791096/
• 关键词: 天疱瘡; デスモグレイン

Pemphigus vulgaris is serious autoimmune blistering disease induced by autoantibodies against desmoglein3 (Dsg3). To make Dsg3 visible, we made a Dsg3-GFP transgenic mouse. When we injected fluorescent labeling anti-Dsg3 antibody into Dsg3-GFP mouse ear skin, we have succeeded in observing the movement of Dsg3 and anti-Dsg3 antibody in vivo. Our observation would be useful to develop the disease-specific therapy to inhibit bulla formation in pemphigus vulgaris in the future.

寻常型天疱疮是由抗粘连蛋白3 （Dsg3）的自身抗体引起的严重自身免疫性水疱病。为了使Dsg3可见，我们制作了Dsg3- gfp转基因小鼠。我们将荧光标记抗Dsg3抗体注射到Dsg3- gfp小鼠耳皮中，成功观察到Dsg3和抗Dsg3抗体在体内的运动情况。本研究结果将为今后发展特异性治疗以抑制寻常型天疱疮大疱形成提供参考。