バクテロデスフィラムに位置する細菌の菌体外蛋白質分泌機構の解明

牙龈卟啉单胞菌是一种口腔厌氧革兰氏阴性菌，与牙周疾病密切相关。细胞外和细胞表面的蛋白水解酶的大部分活性来源于半胱氨酸蛋白酶，称为牙龈痛。我们在类杆菌门的细菌中发现了一种新的蛋白质分泌系统，它负责分泌毒力因子，如牙周病原体牙龈卟啉单胞菌产生的牙周炎蛋白水解酶，并与类杆菌的滑动运动有关，这与其他已有研究的细菌运动形式不同。

Porphyromonas gingivalis is an oral anaerobic Gram-negative bacterium, which is implicated in periodontal pathogenesis. Most of proteolytic activity of extracellular and cell-surface proteinase are derived from cysteine proteinase named gingipains. We found a novel protein secretion system in bacteria of the phylum Bacteroidetes, which is responsible for secretion of virulence factors such as gingipain proteinases produced from the periodontal pathogen Porphyromonas gingivalis and is linked to bacteroidete gliding motility that is distinct from other well-studied forms of bacterial movement.