Effect of unbalanced diet on expression of gut disease-associated gene and intestinal microflora

不均衡饮食对肠道疾病相关基因及肠道菌群表达的影响

• 批准号: 21700757
• 负责人: ARIMOCHI Hideki
• 金额: $ 2.75万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21700757/
• 关键词: 腸内細菌; 大腸癌細胞; サイトカイン; 腸内菌; 腸疾患; 大腸癌; Bacteroides属; マトリックスメタロプロテアーゼ

Bacteroides fragilis, B.vulgatus, and B.thetaiotaomicron were attached on colon tumor cells, then matrix metalloprotease and cytokines produced by the cells were determined. These bacteria could induce these proteins but the level and the pattern of the proteins was quietly different. The differences of the expression pattern were also observed after challenging with distinct strains in one species of bacteria. These results suggest that proteins associated with development of intestinal disease are affected by bacteria attached on intestinal epithelial cells, and their expression pattern is very complicated.

将脆弱拟杆菌（Bacteroides fragile）、普通拟杆菌（b.s vulgatus）和大肠杆菌（b.s thetaiotaomicron）附着在结肠肿瘤细胞上，测定细胞产生的基质金属蛋白酶和细胞因子。这些细菌可以诱导这些蛋白质，但蛋白质的水平和模式是不同的。在同一种细菌中，用不同菌株攻毒后，也观察到表达模式的差异。这些结果表明，与肠道疾病发生相关的蛋白质受到附着在肠上皮细胞上的细菌的影响，其表达模式非常复杂。

项目成果

Production of apoptosis-inducing substances from soybean protein by Clostridium butyricum: Characterization of their toxic effects on human colon carcinoma cells

• DOI: 10.1016/j.canlet.2008.12.006
• 发表时间: 2009-05-18
• 期刊: CANCER LETTERS
• 影响因子: 9.7
• 作者: Arimochi, Hideki;Morita, Kyoji;Kuwahara, Tomomi
• 通讯作者: Kuwahara, Tomomi