Regulation of chondrocyte differentiation through G protein signal

PTHrP信号通过GαS和GαQ在软骨细胞分化调控中发挥重要作用，但具体机制尚不清楚。在本研究中，我们试图建立GαS或GαQ在软骨细胞中过表达的小鼠模型，并建立软骨细胞中GαS或GαQ的条件性基因敲除小鼠，以探讨GαS和GαQ在软骨细胞中的具体作用。此外，我们还试图找到任何与PTHrP受体或其信号下游直接相互作用的蛋白质，并研究了任何参与软骨细胞分化的因素。

PTHrP signal through both G alpha s and G alpha q play important roles in regulation of chondrocyte differentiation, although a precise mechanism is not clear. In this study, we have tried to establish mouse model of both overexpression of either G alpha s or G alpha q in chondrocyte and also establish conditional knock-out mice of either G alpha s or G alpha q in chondrocytes to explore the specific roles of G alpha s and G alpha q in chondrocytes. Moreover, we have explored to find any proteins that interact directly with PTHrP receptor or downstream of its signal and examined any involvement in chondrocyte differentiation.