The elucidation of the relationship between vascular smooth muscle contraction and cytoskeletal rearrangement by proteomics and functional analysis

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Abnormal vascular smooth muscle (VSM) contraction induces acute and fatal diseases such as angina pectoris, myocardial infarction and cerebral infarction. Using focused proteomics, we previously identified several cytoskeleton-related proteins as the candidates for novel signaling molecules mediating abnormal VSM contraction. In the present study, we identified the phosphorylation sites of those proteins. Furthermore, to examine if those cytoskeleton-related proteins were truly involved in abnormal VSM contraction, we developed a high throughput screening system utilizing RNAi and automated cell imaging device, and successfully narrowed down those candidate proteins.