Biological relevance of Coenzyme Q10 binding protein.-Study with prosaposin knockout animal and cell lines-

辅酶Q10(CoQ10)是三磷酸腺苷生物合成所必需的。它也是一种抗氧化剂。皂苷B(Saposin B，SapB)是一种辅酶Q10结合蛋白，对SapB基因敲除小鼠的分析表明，同型动物血浆和肝脏中外源辅酶Q10的浓度显著降低。建立了PSAP转染体(Tf)和基因敲除(KD)细胞系。辅酶Q10含量降低的顺序为：Tf&Gt；亲本&Gt；KD。这些数据表明，SapB和/或其前体PSAP是CoQ10含量的调节器。

Coenzyme Q10 (CoQ10) is necessary for ATP biosynthesis. It also is an antioxidant. Saposin B (SapB) is a CoQ10 binding protein.Analysis using prosaposin (Psap, precursor of SapB) knockout mouse revealed that concentrations of exogenous CoQ10 in plasma and liver were significantly reduced in homo-type animal. Psap transfectant (Tf) and knockdown (KD) HepG2 cells are established. CoQ10 contents decreased as follows: Tf > parent > KD. These data imply that SapB and/orits precursor Psap is a modulator for CoQ10 contents.