DEVELOPMENT OF A CROSSLINKING-BASED ASSAY FOR CHROMATIN RESEARCH
DEVELOPMENT OF A CROSSLINKING-BASED ASSAY FOR CHROMATIN RESEARCH
批准号:
22510219
负责人:
ISHIHARA Satoru
金额:
$2.33万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2012
中文摘要
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英文摘要
The treatment of chromatin with formaldehyde makes nucleosomesto be cross-linked to each other. When there are more nucleosomes in the vicinity, thecross-linking reaction occurs well to produce larger particles including the nucleosomes.These particles can be fractionated in the sedimentation velocity centrifugation, inwhich nucleosomes cross-linked well are separated from ones cross-linked poorly. Here,the fractionation (designated as the SEVENS assay) was developed for chromatin researchapplicable to all mammalian cell types.
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DOI:
10.4049/jimmunol.1003173
发表时间:
2011-09-15
期刊:
Journal of immunology (Baltimore, Md. : 1950)
影响因子:
--
作者:
[Ishihara S, Schwartz RH]
通讯作者:
Schwartz RH
DOI:
10.4049/jimmunol.1201476
发表时间:
2012-11-01
期刊:
Journal of immunology (Baltimore, Md. : 1950)
影响因子:
--
作者:
[Pandiyan P, Yang XP, Saravanamuthu SS, Zheng L, Ishihara S, O'Shea JJ, Lenardo MJ]
通讯作者:
Lenardo MJ
DOI:
10.1093/nar/gkq203
发表时间:
2010-06
期刊:
Nucleic acids research
影响因子:
14.9
作者:
[Ishihara S, Varma R, Schwartz RH]
通讯作者:
Schwartz RH
Two-step binding of transcription factors causes seguential chromatin structural changes at the activated IL-2 promoter
转录因子的两步结合导致激活的 IL-2 启动子处的连续染色质结构变化
DOI:
--
发表时间:
2011
期刊:
The Journal of Immunology
影响因子:
--
作者:
[R.Okazaki, Y.Michikawa, et al, Satoru Ishihara]
通讯作者:
Satoru Ishihara
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Congrains A, Kamide K, Katsuya T, Yasuda O, Oguro R, Yamamoto K, Ohishi M, Rakugi H., 琴村直恵,原田信広,石原悟]
通讯作者:
琴村直恵,原田信広,石原悟
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