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DEVELOPMENT OF A CROSSLINKING-BASED ASSAY FOR CHROMATIN RESEARCH

DEVELOPMENT OF A CROSSLINKING-BASED ASSAY FOR CHROMATIN RESEARCH
开发基于交联的染色质研究检测方法
批准号:
22510219
负责人:
ISHIHARA Satoru
金额:
$2.33万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2012

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中文摘要
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英文摘要
The treatment of chromatin with formaldehyde makes nucleosomesto be cross-linked to each other. When there are more nucleosomes in the vicinity, thecross-linking reaction occurs well to produce larger particles including the nucleosomes.These particles can be fractionated in the sedimentation velocity centrifugation, inwhich nucleosomes cross-linked well are separated from ones cross-linked poorly. Here,the fractionation (designated as the SEVENS assay) was developed for chromatin researchapplicable to all mammalian cell types.
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会议论文
DOI: 10.4049/jimmunol.1003173
发表时间: 2011-09-15
期刊: Journal of immunology (Baltimore, Md. : 1950)
影响因子: --
作者: [Ishihara S, Schwartz RH]
通讯作者: Schwartz RH
DOI: 10.4049/jimmunol.1201476
发表时间: 2012-11-01
期刊: Journal of immunology (Baltimore, Md. : 1950)
影响因子: --
作者: [Pandiyan P, Yang XP, Saravanamuthu SS, Zheng L, Ishihara S, O'Shea JJ, Lenardo MJ]
通讯作者: Lenardo MJ
DOI: 10.1093/nar/gkq203
发表时间: 2010-06
期刊: Nucleic acids research
影响因子: 14.9
作者: [Ishihara S, Varma R, Schwartz RH]
通讯作者: Schwartz RH
Two-step binding of transcription factors causes seguential chromatin structural changes at the activated IL-2 promoter
转录因子的两步结合导致激活的 IL-2 启动子处的连续染色质结构变化
DOI: --
发表时间: 2011
期刊: The Journal of Immunology
影响因子: --
作者: [R.Okazaki, Y.Michikawa, et al, Satoru Ishihara]
通讯作者: Satoru Ishihara
15
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