Identification of a novel binding protein for the (pro)renin receptor

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The (pro)renin receptor (PRR) is a single transmembrane receptor for the pressor enzyme renin and its inactive precursor prorenin. PRR induces the catalytic activation of prorenin along with intracellular signaling. In this study, the glutathione S transferase-fused extracellular domain of PRR expressed in mammalian cells was observed by immunofluorescence microscopy to be localized in the endoplasmic reticulum-like membranous structure, and recovered in the detergent phase in detergent-based two-phase separation experiments, suggesting retention inside the cell through stable membrane association. By co-immunoprecipitation of FALG-tagged PRR with its interacting proteins in human cultured cells and mass spectrometry, GRP78/BiP was identified as a novel binding protein for PRR.