Development of a new probe of a flavonoid metabolite, DOPAC, for understanding the biomolecule modification

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The interest in fermented products in the large intestine such as a quercetin glucoside metabolite, 3,4-dihydroxyphenylacetic acid (DOPAC), has started to grow rapidly. In this study, we identified several plausible target molecules for polyphenols by understanding the molecular mechanisms underlying biological activities related to their chemical characteristics. We also revealed that DOPAC exhibited radical scavenging and phase II drug metabolizing enzyme-inducing activities more potently than other quercetin metabolites. Furthermore, we developed a new probe (DOPAC propargyl ester) to detect target proteins and confirmed its applicability to “click chemistry”.