Development of transgenic animals using a human artificial chromosome (HAC) vector

我们用含有绿色荧光蛋白(GFP)的人人工染色体(HAC)载体建立了猫成纤维细胞的两个细胞系。将转基因细胞插入去核的猫卵中，就产生了克隆胚胎。短时间融合组(30μs)克隆胚胎发育到囊胚的能力高于长时间融合组(60μs)。这些胚胎被移植给了13个受体。只有一名受试者怀孕并产下了一只活猫(74克)。

We established the two cell lines of cat fibroblasts with a human artificial chromosome (HAC) vector containing the green fluorescent protein (GFP). By inserting the transgenic cell into an enucleated cat egg, the cloned embryos were produced. The ability for cloned embryos to develop to blastocysts was higher in the short-time fusion group (30 μsec) than in the long-time fusion group (60 μsec). The embryos were transferred to 13 recipients. Only one recipient became pregnant and delivered a live kitten (74g).