Cellular and Immune Responses of Dairy Cows Induced by Intramammary Infusion of Lactic Acid Bacteria and Therapeutic Trials to Quarters of Cows with Mastitis.

本研究旨在探讨乳内输注乳酸菌（LAB）对奶牛乳腺细胞和免疫功能的影响，以及对奶牛乳腺炎的治疗效果。在输注后24-48小时，在输注的乳区发现SCC显著增加。奶牛的免疫球蛋白，抗菌蛋白和细胞因子的mRNA表达的浓度显着增加的牛奶白细胞。在治疗试验中，乳酸菌对乳区的IMI导致乳腺中显著的细胞和免疫应答。乳酸菌的IMI可能对乳腺炎的控制有一定作用。(key关键词：乳酸菌，乳腺防御，免疫治疗试验，牛乳腺炎控制）

The aim of this study was to characterize the cellular and immune responses of mammary gland by intramammary infusion (IMI) of Lactic acid bacteria (LAB) in cows, and to evaluate the therapeutic effects of IMI of LAB to lactating cows withmastitis. Marked increase of SCC were found in infused quarter at 24-48 hr post infusion. Significantly increased concentrations of immunoglobulins, anti-bacterial proteins and mRNA expression of cytokines on milk leukocytes were found in cows. In therapeutic trials, IMI of LAB to quarters leads to a marked cellular and immune responses in mammary gland. The IMI of LAB may have a possible rolefor control of mastitis.(key words:lactic acid bacteria, mammary gland defense, immune-therapeutic trial, bovine mastitis control)