Development of prevention and treatment methods for infection by enterohemorrhagic Escherichia coli O157 based on bio-protect ive mechanisms

基于生物防护机制的肠出血性大肠埃希菌O157感染防治方法的开发

• 批准号: 22590256
• 负责人: KOBAYSHI Hideyuki
• 金额: $ 2.91万
• 依托单位: University of Occupational and Environmental Health, Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22590256/
• 关键词: 腸管出血性大腸菌; ベロ毒素; 感染症; ワクチン; BCG; 遺伝子組み換え; 生理活性ペプチド; α抗原; 生体防御機構; 脳微小血管

We have developed a novel vaccine using a recombinant BCG expressing the verotoxin B-subunit. The immunized mice with this vaccine survived longer than the nonvaccinated mice, confirminging the validity of this vaccine.On the other hand, the expression of various bioactive peptides in the brain microvascular endothelial cells was changed by verotoxin. The expression of cytokines was increased suggesting that the interaction between brain microvascular endothelial cells and the leukocytes was changed by verotoxin.

我们已经开发了一种新的疫苗，使用表达verotoxin B亚单位的重组BCG。用该疫苗免疫的小鼠比未免疫的小鼠存活时间更长，证实了该疫苗的有效性。另一方面，verotoxin改变了脑微血管内皮细胞中多种生物活性肽的表达。细胞因子的表达增加，表明脑微血管内皮细胞和白细胞之间的相互作用被改变verotoxin。

项目成果

結核菌／BCGのストレプトマイシン耐性・依存性に関する新たな知見．

关于链霉素耐药性和结核分枝杆菌/卡介苗依赖性的新发现。

• 发表时间: 2012
• 作者: Dohgu S;Sumi N;Nishioku T;Takata F;Watanabe T;Naito M;Shuto H;Yamauchi A;Kataoka Y.;藍原大甫,松末公彦,瀧口総一,山野茂;喜多紗斗美;佐藤法仁，阿戸学，黒田誠，山崎利雄，松村隆之，関塚剛史，中山真彰，井上哲圭，本田尚子，土田耕三，小林和夫，大原直也
• 通讯作者: 佐藤法仁，阿戸学，黒田誠，山崎利雄，松村隆之，関塚剛史，中山真彰，井上哲圭，本田尚子，土田耕三，小林和夫，大原直也

Lipidphenotype of two distinct subpopulations of Vayo ohaoteri um bovis Bacillus Calmette-Guerin Tokyo 172 substrain

Vayo ohaoteri um bovis 卡介苗东京 172 亚株两个不同亚群的脂质表型

• 发表时间: 2011
• 期刊: Biol. Chem
• 作者: Naka T.;Maeda S.;Niki M.;Ohara N.;Yamamoto S.,Yano I.,Maeyama J.,Ogura H.;Kobayashi K.,Fujiwara N
• 通讯作者: Kobayashi K.,Fujiwara N

Investigation of acute encephalopathy caused by Shiga toxin 2c-producing enterohemorrhagic E. coli O157 in mice

产志贺毒素2c肠出血性大肠杆菌O157引起小鼠急性脑病的研究

• 发表时间: 2012
• 作者: Fujii J;Amran MY;Yoshida S
• 通讯作者: Yoshida S

Effectiveness azithromycin and/or Chinese medicine in the early and late stage of Shiga toxin-producing Escherichia coli-infected mouse models

阿奇霉素和/或中药对产志贺毒素大肠杆菌感染小鼠模型早期和晚期的有效性

• 发表时间: 2012
• 作者: Amran MY;Fujii J;Yoshida S
• 通讯作者: Yoshida S

Current progress in tuberculosis and recombinant BCG vaccines

结核病和重组卡介苗疫苗的最新进展

• 发表时间: 2012
• 期刊: J. Oral. Biosci
• 作者: Matsui Y;Satoh K;Miyazaki T;Shirabe S;Atarashi R;Mutsukura K;Satoh A;Kataoka Y;Nishida N.;Ohara N
• 通讯作者: Ohara N