Mechanistic study for the hemophagocytosis-like condition during the induction of hematopoietic cells from human pluripotent stem cells

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By using neutrophil-inducing differentiation culture system of human embryonic stem (ES) cells, we observed macrophage-dominant induction of human induced pluripotent stem (iPS) cells. During the early phase of differentiation of human iPS cells, we observed neutrophil-progenitors and the phagocytosis of these progenitors by macrophage, suggesting that our iPS system represent in vitro model of hemophagocytosis known in human diseases. In the present study, we performed molecular analysis of this in vitro model of hemophagocytosis. We observed expression of IFNα1 、IFNα2 、IFNβ1 during the differentiation of all four iPS cell lines and one of three ES cell lines. However, we observed neutrophil-dominant differentiation of human iPS cells under the low cell density culture condition and macrophage-dominant differentiation of human ES cells in certain ES cell lines. Thus, we were not able to clarify the relationship between ES versus iPS cells and neutrophil versus macrophage induction in our differentiation inducing culture system in this study.