Epigenetic mechanism during remyelination in genetic demyelinating mouse model, Twitcher.

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Oligodendrocytes (OLs), the myelinating cell in the CNS, play an important role in brain development and neuronal function. It was revealed that histone deacetylases (HDACs) play a crucial role in the actual generation and differentiation of early oligodendrocytes. In this study, we examined whether the epigenetic program play a role in the genetic demyelinating mouse model, Twitcher. We conducted immunocytochemistry to know the expression of HDAC1 and DNA methyltransferase (DMNT) in the normal and Twitcher mouse brain at postnatal day 20,30, and 40. HDAC1 positive OLs were increased in number in the brain of Twitcher at PND 30, in which demyelination and remyelynation are obvious. HDAC1 positive OLs were seen especially in granular cell layer of cerebellum. Immunocytochemistry did not show any difference of DMNT3 positive cells in both mouse brains.