Acceleration of photoreceptor induction from human iPSCs with gene transfer.

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I investigated the possibility of gene transfer in promoting effect in photoreceptor differentiation in order to shorten the culture period of photoreceptor induction from ESCs/iPSCs. Despite various examinations, the original purpose was pending, however, in the investigation process, Nrl-EGFP mouse iPSCs that expressed EGFP in photoreceptors specifically were produced to detect the effect of gene transfer, and the effect of an inhibitor in retinoic acid signaling to elevate the efficiency of retinal progenitor induction was developed. These research products have expected to work as powerful research tools in other studies of retinal regenerative medicine.