Research of cementum and bone formation in phosphate pyrophosphate imbalance condition

磷酸焦磷酸失衡条件下牙骨质与骨形成的研究

• 批准号: 22791764
• 负责人: SATO Sunao
• 金额: $ 2.58万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22791764/
• 关键词: リン酸; ピロリン酸; セメント質; 骨; PC-1遺伝子; PC-1

The aim of this study was to examine the effect of Pi/ PPi imbalance on gene expression in tissues associated with periodontal tissues, i. e., osteoblasts and cementoblasts, in vivo. Incisors and molars obtained from mice with PC-1 mutations were examined at the light microscopic level with immunohistochemical staining. Histological observations indicated that not only the molars but also the incisors from PC-1 mutant mice showed a marked increase in the amount of cementum when compared to wild-type. Immunohistochemical observation indicated that cementoblasts in wild-type showed high PC-1 expression compared to osteoblast and osteoblasts in PC-1 mutant mice showed high Osteopontin expression compared to wild-type osteoblast. These results suggest that functional changes in proteins such as PC-1, by affecting Pi/ PPi ratio, may in turn affect mineralized tissue formation by altering expression of key genes and associated proteins in osteoblasts and cementoblasts, and further, may impact differentiation potential in follicle cells.

本研究的目的是研究Pi/ PPi失衡对牙周组织相关组织基因表达的影响。例如，成骨细胞和成牙骨质细胞。从PC-1突变的小鼠获得的切牙和磨牙在光镜水平上用免疫组织化学染色进行检查。组织学观察表明，PC-1突变小鼠的臼齿和门牙的牙骨质数量与野生型相比都有显著增加。免疫组化结果显示，与成骨细胞相比，野生型成牙骨质细胞中PC-1的表达更高;与野生型成骨细胞相比，PC-1突变小鼠成骨细胞中骨桥蛋白的表达更高。这些结果表明，蛋白质如PC-1的功能变化，通过影响Pi/ PPi的比例，可能会反过来影响矿化组织的形成，通过改变成骨细胞和成牙骨质细胞中的关键基因和相关蛋白的表达，并进一步影响卵泡细胞的分化潜力。

项目成果

リン酸・ピロリン酸バランスの変化による歯周硬組織への影響

磷酸/焦磷酸盐平衡变化对牙周硬组织的影响

• 发表时间: 2010
• 作者: 山元有理、佐藤淳、岸野万伸;ら
• 通讯作者: ら

Effects of inorganic phosphate on the dento-alveolar complex, in situ and in vitro.

无机磷酸盐对牙槽复合体的原位和体外影响。

• 发表时间: 2010
• 作者: Y.Yamamoto;S.Sunao;et al
• 通讯作者: et al