Research of cementum and bone formation in phosphate pyrophosphate imbalance condition

本研究旨在探讨PI/PPI失衡对牙周组织相关组织成骨细胞和成牙骨质细胞基因表达的影响。对PC-1基因突变小鼠的切牙和磨牙进行免疫组织化学染色，光镜下观察。组织学观察表明，与野生型相比，PC-1突变小鼠的磨牙和门牙的牙骨质数量都显着增加。免疫组织化学观察显示，与成骨细胞相比，野生型成牙本质细胞PC-1表达增强，PC-1突变小鼠成骨细胞骨桥蛋白表达增强。这些结果表明，PC-1等蛋白的功能变化可能通过影响PI/PPI比值，进而通过改变成骨细胞和成牙骨质细胞中关键基因和相关蛋白的表达来影响矿化组织的形成，进而影响毛囊细胞的分化潜能。

The aim of this study was to examine the effect of Pi/ PPi imbalance on gene expression in tissues associated with periodontal tissues, i. e., osteoblasts and cementoblasts, in vivo. Incisors and molars obtained from mice with PC-1 mutations were examined at the light microscopic level with immunohistochemical staining. Histological observations indicated that not only the molars but also the incisors from PC-1 mutant mice showed a marked increase in the amount of cementum when compared to wild-type. Immunohistochemical observation indicated that cementoblasts in wild-type showed high PC-1 expression compared to osteoblast and osteoblasts in PC-1 mutant mice showed high Osteopontin expression compared to wild-type osteoblast. These results suggest that functional changes in proteins such as PC-1, by affecting Pi/ PPi ratio, may in turn affect mineralized tissue formation by altering expression of key genes and associated proteins in osteoblasts and cementoblasts, and further, may impact differentiation potential in follicle cells.