A buildup approach to the design of a small-sized protein via an autonomously-folded short segment

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This study established a methodology for the design of small-sized proteins on the basis of segment elongation by using chignolin that was introduced as a structural support. Using T7 phage displayed library, we selected artificial proteins with affinity for antibody Fc region that was used as a model target. Function and three dimensional structures of artificial proteins were analyzed in detail by using surface plasmon resonance, NMR, and X-ray crystallography.