Analysis of replication initiation systems regulated through the proteins-interacting domain of DnaA

点击翻译按钮获取中文摘要

The initiation of chromosomal replication is strictly controlled to ensure that chromosomal DNA is replicated only once per cell cycle in all cellular organisms. In Escherichia coli, the DnaA initiator is inactivated to repress extra initiations by the Hda-replicase clamp complex. Here, this study showed that specific amino acid residues of DnaA Nand C-terminal domains are important for interaction with Hda. Moreover, candidates of novel initiation regulation factors were searched by protein purification methods, and then identified by mass spectrometry analyses.