Species-specific transport systems for nucleobases and urate in small intestine.

小肠中核碱基和尿酸盐的物种特异性转运系统。

• 批准号: 22790165
• 负责人: INOUE Katsuhisa
• 金额: $ 2.58万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790165/
• 关键词: 核酸塩基; 担体輸送; トランスポーター; SNBT1; 尿酸; 吸収; SLC23A

Nucleobases are important compounds that constitute nucleosides and nucleic acids. Although it has long been suggested that specific transporters are involved in their intestinal absorption, their molecular entities have not been identified in mammals. In the presence study, we could successfully identify rat Slc23a4 as the first sodium dependent nucleobase transporter(SNBT1), which is highly and only expressed in rat small intestine. When transiently expressed in HEK293 cells, rat SNBT1 could transport nucleobases such as uracil, thymine, guanine, hypoxanthine and xanthine, as well as urate. It was also found that the gene orthologous to the rSNBT1 gene is genetically defective in humans. This may have a biological and evolutional meaning in the transport and metabolism of nucleobases and urate.

核碱基是构成核苷和核酸的重要化合物。尽管长期以来一直认为特定的转运蛋白参与其肠道吸收，但尚未在哺乳动物中鉴定出其分子实体。在存在研究中，我们成功地鉴定了大鼠Slc23a4为第一个钠依赖性核碱基转运蛋白（SNBT 1），该蛋白仅在大鼠小肠中高度表达。当在HEK293细胞中瞬时表达时，大鼠SNBT 1可以转运尿嘧啶、胸腺嘧啶、鸟嘌呤、次黄嘌呤和黄嘌呤等核碱基以及尿酸盐。还发现rSNBT1基因的直向同源基因在人类中是遗传缺陷的。这可能在核碱基和尿酸盐的转运和代谢中具有生物学和进化意义。

项目成果

Identification and functional characterization of the first nucleobase transporters in mammals : implication in the species difference in the intestinal absorption mechanism of nucleobases and their analogs between higher primates and other mammals.

哺乳动物中第一个核碱基转运蛋白的鉴定和功能表征：高等灵长类动物和其他哺乳动物之间核碱基及其类似物的肠道吸收机制的物种差异。

• 发表时间: 2010
• 期刊: J.Biol.Chem.
• 作者: Syunsuke;S.;et al.
• 通讯作者: et al.

Identification and functional characterization of the first nucleobase transporter in mammals : implication in the species difference in the intestinal absorption mechanism of nucleobases and their analogs between higher primates and other mammals

哺乳动物中第一个核碱基转运蛋白的鉴定和功能表征：高等灵长类动物和其他哺乳动物之间核碱基及其类似物的肠道吸收机制的物种差异的含义

• 发表时间: 2010
• 期刊: J Biol Chem
• 影响因子: 4.8
• 作者: Yamamoto S;Inoue K;Murata T;Kamigaso S;Yasujima T;Maeda JY;Yoshida Y;Ohta K;Yuasa H
• 通讯作者: Yuasa H

Functional Characteristics of the Human Ortholog of Riboflavin Transporter 2 and Riboflavin-Responsive Expression of Its Rat Ortholog in the Small Intestine Indicate Its Involvement in Riboflavin Absorption

• DOI: 10.3945/jn.110.128330
• 发表时间: 2010-10-01
• 期刊: JOURNAL OF NUTRITION
• 影响因子: 4.2
• 作者: Fujimura, Misaki;Yamamoto, Syunsuke;Yuasa, Hiroaki
• 通讯作者: Yuasa, Hiroaki

Urate transport by SNBT1 : implication in the intestinal disposition of urate

SNBT1 的尿酸盐转运：对尿酸盐肠道处置的影响

• 发表时间: 2012
• 作者: Nishijima C;Inoue K;et al
• 通讯作者: et al

Molecular mechanism of intestinal nucleobase transport in mammals.

哺乳动物肠道核碱基转运的分子机制。

• 发表时间: 2011
• 作者: Inoue;K.;et al.
• 通讯作者: et al.