Analysis in activation of hepatic stem/progenitor cells and development of application to hepatic tissue engineering

肝干/祖细胞活化分析及其在肝组织工程中的应用进展

• 批准号: 22790385
• 负责人: ICHINOHE Norihisa
• 金额: $ 2.5万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22790385/
• 关键词: 細胞・組織; 再生医学; 肝幹・前駆細胞; 生体組織工学; 細胞移植; 分化; 肝幹細胞

Oval cells and small hepatocytes(SHs) are known as hepatic stem and progenitor cells, and Thy1 and CD44 are used as a marker, respectively. Although oval cells are believed to differentiate into mature hepatocytes(MHs) through SHs, the details of their differentiation process are not well understood. In the present experiment we used Thy1^+ oval and CD44^+cells isolated from D-galactosamine-treated rat livers. (1) EGF, basic FGF, or HGF could trigger the hepatic differentiation of sorted Thy1^+ cells to form epithelial cell colonies. (2) Thy1^+oval and CD44^+cells are able to differentiate into hepatocytes, the degree of hepatic maturation of the induced hepatocytes may not be equal to that of normal resident ones. (3) Thy1^+, CD44^+cells and MHs were transplanted into livers treated with retrorsine following partial hepatectomy. Both stem and progenitor cells could differentiate into hepatocytes in host livers. Repopulation efficiency and the survival period of Thy1^+, CD44^+ cells in the long term was very low. The short life of the cells may be due to their cellular senescence.

卵圆细胞和小肝细胞（SH）被称为肝干细胞和祖细胞，Thy 1和CD 44分别被用作标记物。虽然卵圆细胞被认为是通过SH分化为成熟肝细胞（MH），但其分化过程的细节还不清楚。在本实验中，我们使用了从D-半乳糖胺处理的大鼠肝脏中分离的Thy 1 ^+卵圆细胞和CD 44 ^+细胞。(1)EGF、碱性FGF或HGF可触发分选的Thy 1 ^+细胞向肝分化，形成上皮细胞集落。(2)Thy 1 ^+卵圆细胞和CD 44 ^+细胞可分化为肝细胞，诱导肝细胞的肝成熟程度可能与正常肝细胞不同。(3)将Thy 1 ^+、CD 44 ^+细胞和MH移植到部分肝切除后用反曲马新处理的肝脏中。干细胞和祖细胞均可在宿主肝脏中分化为肝细胞。Thy 1 ^+、CD 44 ^+细胞的再增殖效率和长期存活时间均很低。细胞寿命短可能是由于它们的细胞衰老。

项目成果

肝前駆細胞移植治療におけるレシピエントラット肝再生機序の解析

肝祖细胞移植治疗受体大鼠肝再生机制分析

• 发表时间: 2011
• 作者: 市戸義久;他
• 通讯作者: 他

Growth Ability and Repopulation Efficiency of Transplanted Hepatic Stem Cells, Progenitor Cells, and Mature Hepatocytes in Retrorsine-Treated Rat Livers

• DOI: 10.3727/096368911x580626
• 发表时间: 2012-01-01
• 期刊: CELL TRANSPLANTATION
• 影响因子: 3.3
• 作者: Ichinohe, Norihisa;Kon, Junko;Mitaka, Toshihiro
• 通讯作者: Mitaka, Toshihiro

肝硬変ラットモデルにおける肝細胞移植の有用性についての検討

肝细胞移植在肝硬化大鼠模型中的有效性检验

• 发表时间: 2010
• 作者: 中村幸雄;他
• 通讯作者: 他

各種肝病態における肝オーバル細胞・小型肝細胞の同定再生医療叢書第5巻代謝系臓器

各种肝脏病变中肝卵圆细胞和小肝细胞的鉴定再生医学系列第5卷代谢系统器官

• 作者: 市戸義久;大栄秀和;谷水直樹、三高俊広
• 通讯作者: 谷水直樹、三高俊広

Proliferation and osteogenic differentiation of rat bone marrow stromal cells on bioapatite with different crystalline facets

• DOI: 10.1002/jbm.a.32569
• 发表时间: 2010-05-01
• 期刊: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
• 影响因子: 4.9
• 作者: Ichinohe, Norihisa;Nakano, Takayoshi;Tabata, Yasuhiko
• 通讯作者: Tabata, Yasuhiko