Pre- and post-synaptic cell type dependent propofol effects on GABAergic synaptic transmission in rat insular cortex.

为了研究丙泊酚对单一抑制性突触后电流（uIPSC）的影响是否取决于其突触前和突触后细胞类型而受到差异调节，我们对大鼠岛叶皮层（IC）第V层GABA能中间神经元和锥体（Pyr）细胞进行了多个全细胞膜片钳记录。比较突触前FS和突触前非FS连接，突触前FS连接显示丙泊酚对uIPSC电荷转移的更大增强。在突触前FS连接中，FS→Pyr连接比FS →中间神经元对uIPSC电荷传递的增强作用更大，提示丙泊酚对GABA能突触传递的主要靶点是FS→Pyr连接。

To examine whether propofol effects on unitary inhibitory postsynaptic currents (uIPSCs) are differentially regulated depending on their pre- and postsynaptic cell types, we performed multiple whole-cell patch-clamp recording from layer V GABAergic interneurons and pyramidal (Pyr) cells of rat insular cortex (IC). Comparing the presynaptic FS and presynaptic non-FS connections, the presynaptic FS connections showed larger enhancement of uIPSC charge transfer by propofol. Among the presynaptic FS connections, FS→Pyr connections showed larger enhancement of uIPSC charge transfer thanFS→interneurons, indicating that the primary target of propofol to GABAergic synaptic transmission is FS→Pyr connections.