Pre- and post-synaptic cell type dependent propofol effects on GABAergic synaptic transmission in rat insular cortex.

突触前和突触后细胞类型依赖性丙泊酚对大鼠岛叶皮质 GABA 能突触传递的影响。

• 批准号: 22592264
• 负责人: OI Yoshiyuki
• 金额: $ 2.75万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22592264/
• 关键词: 歯科麻酔学; プロポフォール; 大脳皮質; 抑制性シナプス後電流; propofol; pain; insular cortex; GABA(A) receptor; IPSC; whole-cell patch-clamp; GABA; patch clamp

To examine whether propofol effects on unitary inhibitory postsynaptic currents (uIPSCs) are differentially regulated depending on their pre- and postsynaptic cell types, we performed multiple whole-cell patch-clamp recording from layer V GABAergic interneurons and pyramidal (Pyr) cells of rat insular cortex (IC). Comparing the presynaptic FS and presynaptic non-FS connections, the presynaptic FS connections showed larger enhancement of uIPSC charge transfer by propofol. Among the presynaptic FS connections, FS→Pyr connections showed larger enhancement of uIPSC charge transfer thanFS→interneurons, indicating that the primary target of propofol to GABAergic synaptic transmission is FS→Pyr connections.

为了研究丙泊酚对单一抑制性突触后电流（uIPSC）的影响是否取决于其突触前和突触后细胞类型而受到差异调节，我们对大鼠岛叶皮层（IC）第V层GABA能中间神经元和锥体（Pyr）细胞进行了多个全细胞膜片钳记录。比较突触前FS和突触前非FS连接，突触前FS连接显示丙泊酚对uIPSC电荷转移的更大增强。在突触前FS连接中，FS→Pyr连接比FS →中间神经元对uIPSC电荷传递的增强作用更大，提示丙泊酚对GABA能突触传递的主要靶点是FS→Pyr连接。

项目成果

Insulin facilitates repetitive spike firing in rat insular cortex via phosp hoinositide 3-kinase but not mitogen activated protein kinase cascade.

胰岛素通过磷酸肌醇 3-激酶而不是丝裂原激活的蛋白激酶级联促进大鼠岛叶皮质中的重复尖峰放电。

• 发表时间: 2010
• 期刊: Neuroscience
• 影响因子: 3.3
• 作者: Takei H;Fujita S;Shirakawa T;Koshikawa N;Kobayashi M
• 通讯作者: Kobayashi M

Disruption of programmed masticatory movements in unilateral MPTP-treated monkeys as a model of jaw movement abnormality in Parkinson's disease

• DOI: 10.1007/s00702-012-0768-0
• 发表时间: 2012-08-01
• 期刊: JOURNAL OF NEURAL TRANSMISSION
• 影响因子: 3.3
• 作者: Adachi, Kazunori;Kobayashi, Masayuki;Koshikawa, Noriaki
• 通讯作者: Koshikawa, Noriaki

Involvement of NMDA receptors in the ventrolateral striatum of rats in apomorphine-induced jaw movements

• DOI: 10.1016/j.brainres.2010.01.068
• 发表时间: 2010-03-31
• 期刊: BRAIN RESEARCH
• 影响因子: 2.9
• 作者: Fujita, Satoshi;Kiguchi, Motori;Waddington, John L.
• 通讯作者: Waddington, John L.

Postsynaptic cell type-dependent cholinergic regulation of GABAergic synaptic transmission in rat insular cortex.

大鼠岛叶皮质 GABA 突触传递的突触后细胞类型依赖性胆碱能调节。

• 发表时间: 2010
• 期刊: J Neurophysiol 104
• 作者: Yamamoto K;Koyanagi Y;Koshikawa N;Kobayashi M
• 通讯作者: Kobayashi M

プロポフォールは大脳皮質錐体細胞におけるアルファ周波数帯での発火を促進する.

异丙酚促进大脑皮层锥体细胞中α频段的放电。

• 发表时间: 2012
• 作者: Nakamura T;Tomida M;Ando H Takamata T;Kondo E;Asanuma N.;小柳裕子.
• 通讯作者: 小柳裕子.