Gene transfer techniques for study of songbird brain circuit

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Avian species have provided an attractive model system to explore the development and function of brain circuits. Electroporation-mediated and retrovirus(including lentivirus) vector-mediated gene transfer techniques have so far widely been used for introducing genetic material into avian cells. However, it is still challenging to efficiently transduce avian postmitotic neurons without harming the cells. To overcome this problem, we searched a virus vector suitable for gene transfer into avian neurons, and characterize a novel recombinant virus vector, avian adeno-associated virus(A3V) vector.