Analysis of single cell activity stimulated by femtosecond laser-induced impulsive force

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When an intense infrared femtosecond laser is focused into cell culture medium under microscope, an explosion is induced at the laser focal point through an efficient multi-photon absorption. Since the explosion localizes in the area with order of a few tens micron, a mechanical impulsive force can load on a single cultured animal cell. In this investigation, the femtosecond laser-induced impulsive force was loaded on a single muscle myoblast cells and neurons, and then modulation of the activity was investigated by calcium ion(Ca^<2+>) mobilization. An advantage of this stimulation method is that the force magnitude can flexibly control with the laser intensity and pulse repetition rate. The mechanism of the cell activity modulation by the mechanical stimulation was investigated by this new method.