Analysis of single cell activity stimulated by femtosecond laser-induced impulsive force

飞秒激光脉冲力刺激单细胞活性分析

• 批准号: 22657041
• 负责人: HOSOKAWA Yoichiroh
• 金额: $ 2.19万
• 依托单位: Nara Institute of Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22657041/
• 关键词: フェムト秒レーザー; 衝撃力; 細胞; カルシウムイオン; イメージング

When an intense infrared femtosecond laser is focused into cell culture medium under microscope, an explosion is induced at the laser focal point through an efficient multi-photon absorption. Since the explosion localizes in the area with order of a few tens micron, a mechanical impulsive force can load on a single cultured animal cell. In this investigation, the femtosecond laser-induced impulsive force was loaded on a single muscle myoblast cells and neurons, and then modulation of the activity was investigated by calcium ion(Ca^<2+>) mobilization. An advantage of this stimulation method is that the force magnitude can flexibly control with the laser intensity and pulse repetition rate. The mechanism of the cell activity modulation by the mechanical stimulation was investigated by this new method.

当强红外飞秒激光在显微镜下聚焦到细胞培养基中时，通过高效的多光子吸收在激光焦点处诱发爆炸。由于爆炸集中在几十微米量级的区域，机械冲击力可以加载在单个培养的动物细胞上。在这项研究中，飞秒激光诱导的脉冲力加载在单个肌肉成肌细胞和神经元上，然后通过钙离子（Ca^<2+>）动员来研究活性的调制。这种刺激方法的优点是，力的大小可以灵活地控制激光强度和脉冲重复率。利用这种新方法探讨了机械刺激调节细胞活性的机制。

项目成果

AFM探針に過渡的に誘導されるねじれ運動の解析

AFM 尖端瞬态引起的扭转运动分析

• 发表时间: 2012
• 作者: 竹中将信;細川陽一郎
• 通讯作者: 細川陽一郎

超短パルスレーザ誘起衝撃力による細胞操作とその定量評価

超短脉冲激光冲击力细胞操控及其定量评价

• 发表时间: 2010
• 作者: J.B.Laloe;T.Yang;木村崇;大谷義近;細川陽一郎
• 通讯作者: 細川陽一郎

Local stimulation of cultured myocyte cells by femtosecond laser-induced stress wave

• DOI: 10.1007/s00339-010-5927-2
• 发表时间: 2010-12-01
• 期刊: APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
• 影响因子: 2.7
• 作者: Kuo, Yung-En;Wu, Cheng-Chi;Kao, Fu-Jen
• 通讯作者: Kao, Fu-Jen

Non-contact quantification of laser micro-impulse in water by atomic force microscopy and its application for biomechanics

原子力显微镜对水中激光微脉冲的非接触量化及其在生物力学中的应用

• DOI: 10.1117/12.903239
• 发表时间: 2011
• 期刊: Composites Science and Technology
• 影响因子: 9.1
• 作者: Y. Hosokawa

Noncontact estimation of intercellular breaking force using a femtosecond laser impulse quantified by atomic force microscopy

• DOI: 10.1073/pnas.1006847108
• 发表时间: 2011-02-01
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Hosokawa, Yoichiroh;Hagiyama, Man;Ito, Akihiko
• 通讯作者: Ito, Akihiko