Development of growth inhibition of fish pathogenic virus by RNA aptamers

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The selected RNA aptamers against viral hemorrhagic septicemia virus(VHSV) and hirame rhabdovirus(HIRRV) showed highest specificity and affinity for the respective fish pathogenic virusesHirame natural embryo(HINAE) cells treated with the viruses and the corresponding RNA aptamers showed a decrease in incidence of cytopathic effect(CPE) compared to controls(treated only with virus)Rhodovulum sulfidophilum transformed with genes encoding for VHSV-and HIRRV-aptamers produced their extracellular aptamers on a large scale. When fish was cultured in seawater containing R. sulfidophilum which produces VHSV-RNA aptamers, fish mortality during VHSV challenge decreased. It reveals that the recombinant R. sulfidophilum can be a powerful tool for prevention of virus infection in fish