Development of growth inhibition of fish pathogenic virus by RNA aptamers

筛选出的针对病毒出血性败血症病毒(VHSV)和HIRRV的RNA适配子对各自的鱼类致病病毒显示出最高的特异性和亲和力。经这两种病毒和相应的RNA适配子处理的Hirame自然胚胎(HINAE)细胞与对照(仅用病毒处理)相比，细胞病变(CPE)的发生率降低。当鱼类在含有产生VHSV-RNA适配子的硫杆菌的海水中养殖时，鱼类在VHSV攻击期间的死亡率降低。结果表明，重组嗜硫杆菌可作为预防鱼类病毒感染的有力工具。

The selected RNA aptamers against viral hemorrhagic septicemia virus(VHSV) and hirame rhabdovirus(HIRRV) showed highest specificity and affinity for the respective fish pathogenic virusesHirame natural embryo(HINAE) cells treated with the viruses and the corresponding RNA aptamers showed a decrease in incidence of cytopathic effect(CPE) compared to controls(treated only with virus)Rhodovulum sulfidophilum transformed with genes encoding for VHSV-and HIRRV-aptamers produced their extracellular aptamers on a large scale. When fish was cultured in seawater containing R. sulfidophilum which produces VHSV-RNA aptamers, fish mortality during VHSV challenge decreased. It reveals that the recombinant R. sulfidophilum can be a powerful tool for prevention of virus infection in fish