Development of growth inhibition of fish pathogenic virus by RNA aptamers

RNA适体抑制鱼类病原病毒生长的研究进展

• 批准号: 22658059
• 负责人: AOKI Takashi
• 金额: $ 2.34万
• 依托单位: Tokyo University of Marine Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22658059/
• 关键词: RNAアプタマー; 魚類病原ウイルス; ウイルス性出血性敗血症; ヒラメラブドウイルス病; SELEX法; Rhodovulum sulfidophilum; MarineBiotechnology; ヒラメ; Marine Biotechnology

The selected RNA aptamers against viral hemorrhagic septicemia virus(VHSV) and hirame rhabdovirus(HIRRV) showed highest specificity and affinity for the respective fish pathogenic virusesHirame natural embryo(HINAE) cells treated with the viruses and the corresponding RNA aptamers showed a decrease in incidence of cytopathic effect(CPE) compared to controls(treated only with virus)Rhodovulum sulfidophilum transformed with genes encoding for VHSV-and HIRRV-aptamers produced their extracellular aptamers on a large scale. When fish was cultured in seawater containing R. sulfidophilum which produces VHSV-RNA aptamers, fish mortality during VHSV challenge decreased. It reveals that the recombinant R. sulfidophilum can be a powerful tool for prevention of virus infection in fish

筛选出的针对病毒出血性败血症病毒(VHSV)和HIRRV的RNA适配子对各自的鱼类致病病毒显示出最高的特异性和亲和力。经这两种病毒和相应的RNA适配子处理的Hirame自然胚胎(HINAE)细胞与对照(仅用病毒处理)相比，细胞病变(CPE)的发生率降低。当鱼类在含有产生VHSV-RNA适配子的硫杆菌的海水中养殖时，鱼类在VHSV攻击期间的死亡率降低。结果表明，重组嗜硫杆菌可作为预防鱼类病毒感染的有力工具。

项目成果

Inhibition of hirame rhabdovirus (HIRRV) growth by newly developed RNA aptamers

新开发的 RNA 适体抑制 hirame 弹状病毒 (HIRRV) 生长

• 发表时间: 2012
• 作者: Seong Don Hwang;Porntep Punnarak;Hidehiro Kondo;Ikuo Hirono;Takashi Aoki
• 通讯作者: Takashi Aoki

Growth inhibition of viral hemorrhagic septicemia virus by RNA aptamers

RNA适体对病毒性出血性败血症病毒生长的抑制作用

• 发表时间: 2010
• 作者: Porntep Punnarak;Mudjekeewis D. Santos;Hiromichi Suzuki;Yo Kikuchi;HidehiroKondo;Ikuo Hirono;Takashi Aoki
• 通讯作者: Takashi Aoki

RNA aptamer(s) inhibit the growth of the fish pathogen viral hemorrhagic septicemia virus (VSHV)

RNA适体抑制鱼类病原体病毒性出血性败血症病毒（VSHV）的生长

• 发表时间: 2012
• 期刊: Marine Biotechnology14(6)
• 作者: Punnarak;P.,Santos;M. D.;Hwang;S. D.;Kondo;H.;Hirono;I.;Kikuchi;Y.;and Aoki;T.
• 通讯作者: T.

Virulence inhibition of fish virus by RNA aptamers

RNA适体对鱼类病毒的毒力抑制

• 发表时间: 2011
• 作者: Aoki Takashi;Seong Dong Hwang;Porntep Punnarak;Hidehiro Kondo;Ikuo Hirono
• 通讯作者: Ikuo Hirono