Development of DNA microarray for characterization of gene network systems of fish and shellfish

我们对五叶黄尾对虾、牙鲆、红鳍、斑节对虾、日本小对虾和斑节对虾的表达序列标签进行了分析。我们成功地制备了黄尾对虾、比目鱼、红鳍对虾、库鲁玛对虾和黑虎虾的互补基因芯片，分别由1,034、1,965、997、754和1,282个独特的cDNA组成。这些阵列玻片被用于分析脂多糖(LPS)、刀豆蛋白A(ConA)、聚IC和肽聚糖(PG)免疫刺激过程中的基因表达。这些幻灯片还被用于开发和评估针对鱼类病原体设计的疫苗。此外，与敏感品系相比，耐药品系在感染迟缓爱德华氏菌后，抗药性品系的抗原提呈相关基因的表达上调了2倍。总体而言，我们的研究表明，转录组学如微阵列是研究宿主-病原体关系以及可能用于药物开发的有效生物标记物工具。

We conducted expressed sequence tag analysis of yellowtail Seriola quinqueradiata, Japanese flounder Paralichthys olivaceus, red seabream, Pagrus major, kuruma shrimp Marsupenaeus japonicus and black tiger shrimp Penaeus monodon. We were able to successfully develop complementary DNA (cDNA) microarray slides for yellowtail, Japanese flounder, red seabream, kuruma shrimp and black tiger shrimp consisting of 1,034, 1,965, 997, 754, and 1,282 unique cDNAs, respectively. These array slides were used and were proven to be effective in the analysis of gene expression during immunostimulation with lipopolysaccharides (LPS), concanavaline A (conA), poly IC, and peptidoglycan (PG). These slides were also used in the development and evaluation of vaccines designed against fish pathogens. In addition, For Japanese flounder, resistant strains showed 2-fold up-regulation of antigen presentation-related genes following Edwardsiella tarda infection as compared to sensitive strains. Overall, our study shows that transcriptomics such as microarray is an effective biomarker tool in studying host-pathogen relationships and possibly for drug development.