Virulence, infection, and pathogenicity related genes of fish pathogens

在这项研究中，我们分析了巴斯德毕西达、加氏乳球菌、爱德华西埃拉塔达、Vibrio anguillarum和V. parahaemolyticus的病毒相关基因。基因组分析是用来检测P. piscicida KP 9038中的病毒基因的基因。我们已经确定了大约900个克隆人的两个结局。作为这些克隆人与基因库数据库数据库的比较结果，它确定了13个与毒素有关的基因和6个胶囊和LPS合成基因。一些与病毒有关的基因在体外没有被检测到的特征。These results suggest that P. piscicida may express several virulence related genes only in vivo。Five different clones were isolated from the gene library of the L garvieae SA8201 （KG-） strain by immunological screening using rabbit serum against L. garvieae （KG-） phenotype cells.免疫学上检测到的蛋白质的氨基酸序列与加工蛋白质、二羟基磷酸盐合成酶、触发因子、N-乙酰葡萄糖胺-6-磷酸脱乙酰酶和抗噬细胞蛋白质是同源的。Five genes were specifically expressed in the virulence KG-phenotype.The eth hemolysin gene locus of E. Tarda编码血脂蛋白EthA蛋白及其附属蛋白EthB。我们由站点指向标记插入在以太A或以太B中构建的突变体。Both the ethA y D1-y D1 and ethB y D1-y D1 mutants lacked extracellular， cell-associated， and intracellular Hemolytic activities。这些单基因突变体的病理学比原来的淀粉更弱，来自V. anguillarum KV 9001和V. parahaemolyticus ATCC 17802的衣原体基因被克隆。这些基因中的滥用氨基酸序列有71.6%的身份。真空管基因在V. anguillarum中具有极高的优越性，并将真空管基因杂交与V. Arginolyticus和Beneckea Proteolytica DNA的DNA探针。DNA probe of the vpc gene hybridized to V. alinolyticus， V. harveyi， and V. ordalii DNA。

In this study, we analysed the virulence related genes of Pasteurella piscicida, Lactococcus garvieae, Edwardsiella tarda, Vibrio anguillarum and V. parahaemolyticus.Genome analysis was conducted to detect the virulence genes in P. piscicida KP9038. We have sequenced the both ends of approximately 900 clones. As a result of comparisons of these clones to the GeneBank database, it was determined that there are 13 toxin related genes, and 6 capsule and LPS synthesis genes. Characteristics of some of these virulence related genes have not detected in vitro. These results suggest that P. piscicida may express several virulence related genes only in vivo.Five different clones were isolated from the gene library of the L garvieae SA8201 (KG-) strain by immunological screening using rabbit serum against L. garvieae (KG-) phenotype cells. The amino acid sequences of the immunologically detected proteins were homologous to a processing protease, dihydropteroate synthase, trigger factor, N-acetylglucosamine-6-phosphate deacetylase, and anti-phagocytosis M protein. Five genes were specifically expressed in the virulence KG- phenotype strains.The eth hemolysin gene locus of E. tarda encodes the hemolysin EthA protein and its accessory protein EthB. We constructed mutants by site-directed marker insertion mutagenesis in ethA or ethB. Both the ethAィイD1-ィエD1 and ethBィイD1-ィエD1 mutants lacked extracellular, cell-associated, and intracellular hemolytic activities. The pathogenicity of these single gene mutants became weaker than that of original stain.Chitinase genes from V. anguillarum KV9001 and V. parahaemolyticus ATCC17802 were cloned. The deduced amino acid sequences of these genes have 71.6% identity. The vac gene was highly prevalent in V. anguillarum, and the DNA probe of the vac gene hybridized to V. alginolyticus and Beneckea proteolytica DNA. The DNA probe of the vpc gene hybridized to V. alginolyticus, V. harveyi, and V. ordalii DNA.